Examinando por Autor "Ferreira, Henrique Bunselmeyer"

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    Weighted gene co-expression network analysis reveals immune evasion related genes in Echinococcus granulosus sensu stricto
    (Frontiers Media SA, 2023) Pereira, Ismael; Prado Paludo, Gabriela; Hidalgo, Christian; Stoore, Caroll; Baquedano, María Soledad; Cabezas, Carolina; Cancela, Martín; Ferreira, Henrique Bunselmeyer; Bastías, Macarena; Riveros, Aníbal; Meneses, Claudio; Sáenz, Leonardo; Paredes, Rodolfo
    Cystic echinococcosis (CE) is a zoonotic disease caused by the tapeworm Echinococcus granulosus sensu lato (s.l). In the intermediate host, this disease is characterized by the growth of cysts in viscera such as liver and lungs, inside of which the parasite develops to the next infective stage known as protoscoleces. There are records that the infected viscera affect the development and morphology of E. granulosus s.l. protoscolex in hosts such as buffalo or humans. However, the molecular mechanisms that drive these differences remains unknown. Weighted gene co-expression network analysis (WGCNA) using a set of RNAseq data obtained from E. granulosus sensu stricto (s.s.) protoscoleces found in liver and lung cysts reveals 34 modules in protoscoleces of liver origin, of which 12 have differential co-expression from protoscoleces of lung origin. Three of these twelve modules contain hub genes related to immune evasion: tegument antigen, tegumental protein, ubiquitin hydrolase isozyme L3, COP9 signalosome complex subunit 3, tetraspanin CD9 antigen, and the methyl-CpG-binding protein Mbd2. Also, two of the twelve modules contain only hypothetical proteins with unknown orthology, which means that there are a group of unknown function proteins co-expressed inside the protoscolex of liver CE cyst origin. This is the first evidence of gene expression differences in protoscoleces from CE cysts found in different viscera, with co-expression networks that are exclusive to protoscoleces from liver CE cyst samples. This should be considered in the control strategies of CE, as intermediate hosts can harbor CE cysts in liver, lungs, or both organs simultaneously. Copyright © 2024 Pereira, Paludo, Hidalgo, Stoore, Baquedano, Cabezas, Cancela, Ferreira, Bastías, Riveros, Meneses, Sáenz and Paredes.