Examinando por Autor "Garrido, Daniel"

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    Assessment of changes in the oral microbiome that occur in dogs with periodontal disease
    (MDPI, 2021-12) Santibáñez, Rodrigo; Rodríguez-Salas, Camila; Flores-Yáñez, Carla; Garrido, Daniel; Thomson, Pamela
    The oral microbiome in dogs is a complex community. Under some circumstances, it contributes to periodontal disease, a prevalent inflammatory disease characterized by a complex interaction between oral microbes and the immune system. Porphyromonas and Tannerella spp. are usually dominant in this disease. How the oral microbiome community is altered in periodontal disease, especially sub-dominant microbial populations is unclear. Moreover, how microbiome functions are altered in this disease has not been studied. In this study, we compared the composition and the predicted functions of the microbiome of the cavity of healthy dogs to those with from periodontal disease. The microbiome of both groups clustered separately, indicating important differences. Periodontal disease resulted in a significant increase in Bacteroidetes and reductions in Actinobacteria and Proteobacteria. Porphyromonas abundance increased 2.7 times in periodontal disease, accompanied by increases in Bacteroides and Fusobacterium. It was predicted that aerobic respiratory processes are decreased in periodontal disease. Enrichment in fermentative processes and anaerobic glycolysis were suggestive of an anaerobic environment, also characterized by higher lipopolysaccharide biosynthesis. This study contributes to a better understanding of how periodontal disease modifies the oral microbiome and makes a prediction of the metabolic pathways that contribute to the inflammatory process observed in periodontal disease. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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    Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs
    (PeerJ Inc., 2022-02) Thomson, Pamela; Santibáñez, Rodrigo; Rodríguez-Salas, Camila; Flores-Yañez, Carla; Garrido, Daniel
    Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries. It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases. The intestinal microbiome of obese animals shows increases in the abundance of certain members capable of extracting energy from complex polysaccharides. The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs. Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10). DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3–V4 region and bioinformatics analysis targeting microbiome composition and function. Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs. Microbiome compositions of these animals correlated with their BCS, and obese dogs showed enrichment in pathways related to transport, chemotaxis, and flagellar assembly. These results highlight the differences in the gut microbiome between normal weight and obese dogs and prompt further research to improve animal health by modulating the gut microbiome. Copyright 2022 Thomson et al.
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    Preliminary Functional Analysis of the Gut Microbiome in Colic Horses
    (Multidisciplinary Digital Publishing Institute (MDPI), 2024-11) Thomson, Pamela; Garrido, Daniel; Santibáñez, Rodrigo; Lara, Felipe
    Colic in horses is a common condition that can affect different organs of the abdominal cavity. In recent years, attempts have been made to associate this pathology with changes that occur in the intestinal microbiome. Through a case–control study, we analyzed the intestinal microbiome of a group of healthy horses and another with colic by massive sequencing of 16S rRNA to observe the differences in the bacterial composition and functionality of these groups. The intestinal microbiomes of both groups are dominated by the phyla Firmicuteota, Bacteroidota, and Pseudomonadota. The abundance of Firmicuteota was negatively correlated with Pseudomonadota and Actinobacteriota in horses with colic. The microbiome of equine colic was predicted to be enriched with aerobic respiration pathways and fatty acid and amino acid degradation, observations that indicate discrete but important differences in the intestinal microbiome of horses with colic, which correlate with a more pro-inflammatory microbial community.