Examinando por Autor "Glaeser, Stefanie P."

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    Paracoccus nototheniae sp. nov., isolated from a black rock cod fish (Notothenia coriiceps) from the Chilean Antarctic
    (International Journal of Systematic and Evolutionary Microbiology, 2019) Kampfer, Peter; Irgang, Rute; Poblete-Morales, Matías; Fernandez-Negrete, Guillermo; Glaeser, Stefanie P.; Fuentes-Messina, Derie; Avendaño-Herrera, Ruben
    An orange-pigmented, oxidase-positive bacterial strain (I-41R45T), isolated from the kidney of a black rock cod fish sampled in the Chilean Antarctic was studied in a polyphasic taxonomic investigation. Cells of the isolate were coccoid and stained Gram-negative. A comparison of the 16S rRNA gene sequence of strain I-41R45T with sequences of type strains of most closely related Paracoccus species showed highest sequence similarities to Paracoccus hibiscisoli (98.4 %), Paracoccus marcusii (98.3 %), Paracoccus haeundaensis and Paracoccus carotinifaciens (both 98.2 %). 16S rRNA gene sequence similarities to all other Paracoccus species were below 97 %. The draft genome of strain I-41R45T had a size of 4.59 Mb with a DNA G+C content of 65.26 mol% and included the prediction and annotation of 4426 coding genes, 1973 protein-coding genes and 46 tRNAs. The fatty acid profile of strain I-41R45T consisted mainly of the major fatty acids C18: 1ω7c/ω9t/ω12t and C18:0, typical of the genus Paracoccus. DNA–DNA hybridizations between I-41R45T and type strains of P. hibiscisoli, P. marcusiiand P. haeundaensis resulted in similarity values of 45 % (reciprocal 26 %), 66 % (reciprocal 61 %), and 29 % (reciprocal 36 %), respectively. DNA–DNA hybridization results, together with the differentiating biochemical and chemotaxonomic properties, showed that strain I-41R45T represents a novel Paracoccus species, for which the name Paracoccus nototheniae sp. nov. (type strain I-41R45T=CCM 8875T=CIP 111632T), is proposed.
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    Pseudoduganella danionis sp. nov., isolated from zebrafish (Danio rerio)
    (Microbiology Society, 2016-10) Kämpfer, Peter; Irgang, Rute; Busse, Hans-Jürgen; Poblete-Morales, Mathías; Kleinhagauer, Tanita; Glaeser, Stefanie P.; Avendaño-Herrera, Ruben
    One beige-pigmented, Gram-staining-negative, rod-shaped bacterium, strain E3/2T , was isolated from a zebrafish, Daniorerio. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared 97.7 % 16S rRNA gene sequence similarity to the species Pseudoduganella violaceinigra and between 97.4 to 97.0 % to some species of the genera Duganella and Massilia, including Duganella radicis, Duganella phyllosphaerae, Massilia dura, Massilia lutea, Duganella sacchari, Duganella zoogloeoides, Massilia albidiflava and Massilia umbonata. Sequence similarities to all other species were below 97 %. The main cellular fatty acids of the strain were summed feature 3 fatty acids (C16 : 1!7c/iso-C15 : 0 2-OH), C10 : 0 3-OH, C16 : 0 and C12 : 0. The polyamine pattern of strain E3/2T contained predominantly putrescine and 2-hydroxyputrescine. The major quinone was ubiquinone Q-8. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Based on phylogenetic, chemotaxonomic, genomic and phenotypic analyses we propose a novel species of the genus Pseudoduganella named Pseudoduganella danionis sp. nov., with strain E3/2T (=LMG 29678T=CCM 8698T ) as the type strain.
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    Psychromonas aquatilis sp. nov., isolated from seawater samples obtained in the Chilean Antarctica
    (Microbiology Society, 2017-05) Kämpfer, Peter; Irgang, Rute; Poblete-Morales, Matías; Glaeser, Stefanie P.; Martín, Marcelo Cortez-San; Avendaño-Herrera, Ruben
    A slightly beige-white pigmented, Gram-staining-negative, rod-shaped bacterium, strain M1A1T, was isolated from seawater samples obtained in Fildes Bay, Antarctica (62°12′ S 58° 57′ W). Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the isolate shared 98.4% 16S rRNA gene sequence identity to the type strain of Psychromonas arctica, but less than 97% 16S rRNA gene sequence similarities to all other species of the genus Psychromonas. DNA–DNA hybridization with Psychromonas arctica DSM 14288T showed low values (21%, reciprocal 27%). The main cellular fatty acid of strain M1A1T was summed feature 3 fatty acids (C16: 1ω7c/C16: 1ω8c), followed by C16: 0. Based on phylogenetic, chemotaxonomic, genomic and phenotypic analyses, we propose a novel species of the genus Psychromonas with the name Psychromonas aquatilis sp. nov. and the strain M1A1T (=CIP 111183T=CCM 8710T=LMG 29766T) as type strain. © 2017 IUMS.