Examinando por Autor "Gonzalez, C."

Mostrando 1 - 2 de 2
  • No hay miniatura disponible
    Ítem
    Eggshell membrane as a biodegradable bone regeneration inhibitor
    (WILEY, 2008-06-01) Arias, J. I.; Gonzalez, A.; Fernandez, M. S.; Gonzalez, C.; Saez, D.; Arias, J. L.
    The efficiency of chicken eggshell membranes combined with a minimally invasive small osteotomy procedure of the ulna to accomplish an efficient release of the radius so that it can continue to grow in an unstressed manner was tested in rabbits. Eggshell membranes were extracted from chicken eggs, rinsed, dried and sterilized with ethylene oxide for 24 h. For reactivity testing, four separate subcutaneous pockets were created in 10 rats in the paravertebral region by blunt dissection and eggshell membranes were implanted in two of them. After 1-16 weeks, the implants were retrieved with the surrounding soft tissues and submitted to histological examination. Subsequently, 10 rabbits were anaesthetized and a complete 0.5 mm. wide osteotomy was performed in both the right and the left distal ulna. A piece of eggshell membranes was interposed in the osteotomy site of one ulna. The opposite osteotomized ulna was left as a negative control. The rabbits were injected with oxytetracycline at the time of surgery and this was repeated every 7 days for labelling new bone formation. After 1-16 weeks, ulnar osteotomized regions were histologically examined. After histological, fluorescence microcopy and radiological evaluation, we demonstrate here for the first time that eggshell membranes as interpositional material in rabbit osteotomized ulnar experiments acted as an active barrier against bone bridging. The degradation of the eggshell membrane, due to host reaction, appeared sufficiently late to cause the desirable delay of bone healing that is compatible with the time needed for a corrective response. Copyright (C) 2008 John Wiley & Sons, Ltd.
  • Miniatura
    Ítem
    Genomic analysis of the necrotrophic fungal pathogens sclerotinia sclerotiorum and botrytis cinerea
    (Public Library of Science, 2011) Amselem, J.; Cuomo, C.; van Kan, J.; Viaud, M.; Benito, E.; Couloux, A.; Coutinho, P.; de Vries, R.; Dyer, P.; Fillinge, S.; Fournier, E.; Gout, L.; Hahn, M.; Kohn, L.; Lapalu, N.; Plummer, K.; Pradier, J.; Quévillon, E.; Sharon, A.; Simon, A.; Have, A.; Tudzynski, B.; Tudzynski, P.; Wincker, P.; Andrew, M.; Anthouard, V.; Beever, R.; Beffa, R.; Benoit, I.; Bouzid, O.; Brault, B.; Chen, Z.; Choquer, M.; Collémare, J.; Cotton, P.; Danchin, E.; Da Silva, C.; Gautier, A.; Giraud, C.; Giraud, T.; Gonzalez, C.; Grossetete, S.; Güldener, U.; Henrissat, B.; Howlett, B.; Kodira, Ch.; Kretschmer, M.; Lappartient, A.; Leroch, M.; Levis, C.; Mauceli, E.; Neuvéglise, C.; Oeser, B.; Pearson, M.; Poulain, J.; Poussereau, N.; Quesneville, H.; Rascle, C.; Schumacher, J.; Ségurens, B.
    Sclerotinia sclerotiorum and Botrytis cinerea are closely related necrotrophic plant pathogenic fungi notable for their wide host ranges and environmental persistence. These attributes have made these species models for understanding the complexity of necrotrophic, broad host-range pathogenicity. Despite their similarities, the two species differ in mating behaviour and the ability to produce asexual spores. We have sequenced the genomes of one strain of S. sclerotiorum and two strains of B. cinerea. The comparative analysis of these genomes relative to one another and to other sequenced fungal genomes is provided here. Their 38-39 Mb genomes include 11,860-14,270 predicted genes, which share 83% amino acid identity on average between the two species. We have mapped the S. sclerotiorum assembly to 16 chromosomes and found large-scale co-linearity with the B. cinerea genomes. Seven percent of the S. sclerotiorum genome comprises transposable elements compared to