Examinando por Autor "Ibarra, Pablo"

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    Teaching is a blockbuster: busing authentic materials, specifically film clips, as a didactical tool to reinforce the use of the third person singular structure in Chilean seventh grades EFL students using task-based activities
    (Universidad Andrés Bello, 2012) Araya, María Paz; Bazaes, Matías; Ibarra, Pablo; Troncoso, Ignacio; Popp Ward, Jennie; Facultad de Humanidades y Educación; Escuela de Educación y Ciencias Sociales
    This research paper aims to analyze the likelihood of the effectiveness of incorporating film clips and Task-Based Activities in EFL classrooms as a possible tool to reduce the frequency of third person singular simple present mistakes and errors in students' productive skills. The study was carried out by conducting a quantitative experimental research Study; which consisted on the application of faur types of pre-test and post-test to ali the participants. In addition to the tests, an intervention was made far the intervention group. Furthermore, the experiment was conducted in two elementary grades from a Santiago Centro school. One grade was considered as control group and the other as intervention group; both groups played the role of sample far this research study. The intervention was carried out in faur weeks and it included pre-test, intervention regarding the use of authentic material and task-based activities, in addition to the post-test.
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    Ítem
    View more Major antigenic differences in Aeromonas salmonicida isolates correlate with the emergence of a new strain causing furunculosis in Chilean salmon farms
    (Frontiers Media SA, 2025) Mancilla, Marcos; Ojeda, Adriana; Yuivar, Yassef; Grandón, Maritza; Grothusen, Horst; Oyarzún, Marcela; Bisquertt, Alejandro; Ugalde, Juan A.; Fuentes, Francisco; Ibarra, Pablo; Bustos, Patricio
    Aeromonas salmonicida is the etiological agent of furunculosis, a septicemic disease with high mortality rates affecting salmonids and other teleost species worldwide. Reviewing molecular diagnostic protocols for routine diagnostics, we realized that the amplification of the vapA target gene failed in some cases of furunculosis. Therefore, we hypothesized that the emergence of a new strain may be involved in recent outbreaks. In this work, we demonstrate that the vapA locus is absent in the new strain, which explains why it lacks the major membrane component VapA protein, a critical virulence factor. In addition, we found that the vapA-absent strain differs from its counterparts in outer membrane protein and lipopolysaccharide profiles, suggesting profound changes at the membrane structure level and in antigenic properties. These features along with sequence analysis information allowed us to infer that a complex genomic rearrangement, probably an indel encompassing the entire vapA locus, gave rise to this membrane phenotype. Although the causes for pathogen evolution and emergence were not fully elucidated, our results strongly suggest that the vapA-absent strain is responsible for a raising proportion of recent furunculosis cases, and that it may be related to a less virulent disease and a low serological response upon vaccination with the A. salmonicida antigen formulation currently used in Chile.