Examinando por Autor "Montealegre, Jaime R."
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Ítem Control of grey rot of apple fruits by biologically active natural products(Sociedade Brasileira de Fitopatologia, 2010) Montealegre, Jaime R.; López, Catalina; Stadnik, Marciel J.; Henríquez, José L.; Herrera, Rodrigo; Polanco, Rubén; Di Piero, Robson M.; Pérez, Luz M.Biorend SC (chitosan), BC-1000 EC (grapefruit extract plus bioflavonoids) and ECO-100 SC (bioflavonoids plus organic acids, citric phytoalexins, fatty acids, glycerides and sugars), respectively, suppressed grey rot of apple caused by B. cinerea by 80.1%, 79.0% and 76.5% when used as post-harvest treatments under controlled conditions. When applied as combined pre- and post-harvest treatments Biorend SC inhibited fruit rot by 49.9 %, while BC-1000 EC and ECO-100 SC were ineffective. None of the products inhibited fruit rot when applied as pre-harvest treatments under controlled conditions or as post-harvest treatments under commercial conditions. The algal polysaccharide ulvan used in post-harvest treatments suppressed grey rot by 56.0% under controlled conditions, but had no inhibitory effect on combined pre- and post-harvest treatments. The inability of products to activate defense mechanisms (chitinase and peroxidase) of fruits was consistent with the unsuccessful control of rot by pre-harvest treatment. The results suggest that the natural products used have potential for use in integrated management of Botrytis rot when applied after harvest.Ítem New strains obtained after UV treatment and protoplast fusion of native Trichoderma harzianum: their biocontrol activity on Pyrenochaeta lycopersici(Pontificia Universidad Católica de Valparaíso, 2007) Besoain, Ximena A.; Pérez, Luz María; Araya, Antonio; Lefever, Llacolén; Sanguinetti, Melina; Montealegre, Jaime R.The obtainment of 30 new strains from native Trichoderma harzianum after UV light irradiation (UV-A and UV-C), and of 82 strains resulted from protoplast fusion were accomplished. The new strains, initially selected for their growing rate under low temperature and high pH conditions, as well as for their innocuousness on tomato plants, were tested for in vitro inhibition of Pyrenochaeta lycopersici in dual cultures and due to secretion of volatile and diffusible metabolites. All the UV-A and UV-C selected candidate mutants were innocuous to tomato plants, but none of them showed improvement in their biocontrol activity on P. lycopersici. Th12A20.1 increased 1.3 and 1.9 fold the total fresh weight of Fortaleza tomato plants when compared to its parental strains Th12 and Th11, respectively. The selected candidate mutants obtained through protoplast fusion were also innocuous to tomato plants, but only ThF1-2 and ThF4-4 inhibited 1.3 fold (in dual cultures) and 5 fold (due to secretion of volatile metabolites) the growth of P. lycopersici, respectively, in relation to the mean inhibitory effect of both parents. Therefore, these candidate mutants could be included in experiments under field conditions.