Examinando por Autor "Montenegro, Ivan"

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    Ítem
    Effect of Electrical Stimulation on PC12 Cells Cultured in Different Hydrogels: Basis for the Development of Biomaterials in Peripheral Nerve Tissue Engineering
    (MDPI, 2023-12) Olguín, Yusser; Selva, Mónica; Benavente, Diego; Orellana, Nicole; Montenegro, Ivan; Madrid, Alejandro; Jaramillo-Pinto, Diego; Otero, María Carolina; Corrales, Tomas P.; Acevedo, Cristian A.
    Extensive damage to peripheral nerves is a health problem with few therapeutic alternatives. In this context, the development of tissue engineering seeks to obtain materials that can help recreate environments conducive to cellular development and functional repair of peripheral nerves. Different hydrogels have been studied and presented as alternatives for future treatments to emulate the morphological characteristics of nerves. Along with this, other research proposes the need to incorporate electrical stimuli into treatments as agents that promote cell growth and differentiation; however, no precedent correlates the simultaneous effects of the types of hydrogel and electrical stimuli. This research evaluates the neural differentiation of PC12 cells, relating the effect of collagen, alginate, GelMA, and PEGDA hydrogels with electrical stimulation modulated in four different ways. Our results show significant correlations for different cultivation conditions. Electrical stimuli significantly increase neural differentiation for specific experimental conditions dependent on electrical frequency, not voltage. These backgrounds allow new material treatment schemes to be formulated through electrical stimulation in peripheral nerve tissue engineering. © 2023 by the authors.
  • Miniatura
    Ítem
    Study on the cytotoxic activity of drimane sesquiterpenes and nordrimane compounds against cancer cell lines
    (MDPI AG, 2014) Montenegro, Ivan; Tomasoni, Giacomo; Bosio, Claudia; Quiñones, Natalia; Madrid, Alejandro; Carrasco, Hector; Olea, Andres; Martinez, Rolando; Cuellar, Mauricio; Villena, Joan
    Twelve drimanes, including polygodial (1), isopolygodial (2), drimenol (3), confertifolin (4), and isodrimenin (5), were obtained from natural sources. Semi-synthetic derivatives 6-12 were obtained from 1 and 2, and cytotoxic activity was evaluated in vitro against cancer cell lines (HT-29, MDA-MB231, DHF, MCF-7, PC-3, DU-145, and CoN). IC50 values were determined at concentrations of 12.5-100 ìM of each compound for 72 h. In addition, it was found that polygodial (1), 8, and 12 induced changes in mitochondrial membrane permeability in CoN, MCF-7, and PC-3 cells. © 2014 by the authors.