Examinando por Autor "Moya-Beltrán, Ana"
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Ítem Draft genome sequence of chloride-tolerant Leptospirillum ferriphilum Sp-Cl from industrial bioleaching operations in northern Chile(BIOMED CENTRAL, 2016-02) Issotta, Francisco; Galleguillos, Pedro A.; Moya-Beltrán, Ana; Davis-Belmar, Carol S.; Rautenbach, George; Covarrubias, Paulo C.; Acosta, Mauricio; Ossandon, Francisco J.; Contador, Yasna; Holmes, David S.; Marín-Eliantonio, Sabrina; Quatrini, Raquel; Demergasso, CeciliaLeptospirillum ferriphilum Sp-Cl is a Gram negative, thermotolerant, curved, rod- shaped bacterium, isolated from an industrial bioleaching operation in northern Chile, where chalcocite is the major copper mineral and copper hydroxychloride atacamite is present in variable proportions in the ore. This strain has unique features as compared to the other members of the species, namely resistance to elevated concentrations of chloride, sulfate and metals. Basic microbiological features and genomic properties of this biotechnologically relevant strain are described in this work. The 2,475,669 bp draft genome is arranged into 74 scaffolds of 74 contigs. A total of 48 RNA genes and 2,834 protein coding genes were predicted from its annotation; 55 % of these were assigned a putative function. Release of the genome sequence of this strain will provide further understanding of the mechanisms used by acidophilic bacteria to endure high osmotic stress and high chloride levels and of the role of chloride-tolerant iron-oxidizers in industrial bioleaching operations.Ítem Draft genome sequence of the nominated type strain of "Ferrovum myxofaciens," an acidophilic, iron-oxidizing betaproteobacterium(American Society for Microbiology, 2014) Moya-Beltrán, Ana; Cárdenas, Juan Pablo; Covarrubias, Paulo C.; Issotta, Francisco; Ossandon, Francisco J.; Grail, Barry M.; Holmes, David S.; Quatrini, Raquel; Johnson, D. BarrieFerrovum myxofaciens” is an iron-oxidizing betaproteobacterium with widespread distribution in acidic low-temperature en vironments, such as acid mine drainage streams. Here, we describe the genomic features of this novel acidophile and investigate the relevant metabolic pathways that enable its survival in these environments.Ítem Genomic evolution of the class Acidithiobacillia: deep-branching Proteobacteria living in extreme acidic conditions(Springer Nature, 2021-11) Moya-Beltrán, Ana; Beard, Simón; Rojas-Villalobos, Camila; Issotta, Francisco; Gallardo, Yasna; Ulloa, Ricardo; Giaveno, Alejandra; Degli Esposti, Mauro; D. Barrie, Johnson; Quatrini, RaquelMembers of the genus Acidithiobacillus, now ranked within the class Acidithiobacillia, are model bacteria for the study of chemolithotrophic energy conversion under extreme conditions. Knowledge of the genomic and taxonomic diversity of Acidithiobacillia is still limited. Here, we present a systematic analysis of nearly 100 genomes from the class sampled from a wide range of habitats. Some of these genomes are new and others have been reclassified on the basis of advanced genomic analysis, thus defining 19 Acidithiobacillia lineages ranking at different taxonomic levels. This work provides the most comprehensive classification and pangenomic analysis of this deep-branching class of Proteobacteria to date. The phylogenomic framework obtained illuminates not only the evolutionary past of this lineage, but also the molecular evolution of relevant aerobic respiratory proteins, namely the cytochrome bo3 ubiquinol oxidases. © 2021, The Author(s).Ítem Genomic evolution of the class Acidithiobacillia: deep-branching Proteobacteria living in extreme acidic conditions(2021-11) Moya-Beltrán, Ana; Beard, Simón; Rojas-Villalobos, Camila; Issotta, Francisco; Gallardo, Yasna; Ulloa, Ricardo; Giaveno, Alejandra; Degli Esposti, Mauro; Johnson, D. Barrios; Quatrini, RaquelMembers of the genus Acidithiobacillus, now ranked within the class Acidithiobacillia, are model bacteria for the study of chemolithotrophic energy conversion under extreme conditions. Knowledge of the genomic and taxonomic diversity of Acidithiobacillia is still limited. Here, we present a systematic analysis of nearly 100 genomes from the class sampled from a wide range of habitats. Some of these genomes are new and others have been reclassified on the basis of advanced genomic analysis, thus defining 19 Acidithiobacillia lineages ranking at different taxonomic levels. This work provides the most comprehensive classification and pangenomic analysis of this deep-branching class of Proteobacteria to date. The phylogenomic framework obtained illuminates not only the evolutionary past of this lineage, but also the molecular evolution of relevant aerobic respiratory proteins, namely the cytochrome bo3 ubiquinol oxidases.Ítem Insights into the biology of acidophilic members of the Acidiferrobacteraceae family derived from comparative genomic analyses(Elsevier Masson SAS, 2018-12) Issotta, Francisco; Moya-Beltrán, Ana; Mena, Cristóbal; Covarrubias, Paulo C.; Thyssen, Christian; Bellenberg, Sören; Sand, Wolfgangd; Quatrini, Raquel; Vera, MarioThe family Acidiferrobacteraceae (order Acidiferrobacterales) currently contains Gram negative, neutrophilic sulfur oxidizers such as Sulfuricaulis and Sulfurifustis, as well as acidophilic iron and sulfur oxidizers belonging to the Acidiferrobacter genus. The diversity and taxonomy of the genus Acidiferrobacter has remained poorly explored. Although several metagenome and bioleaching studies have identified its presence worldwide, only two strains, namely Acidiferrobacter thiooxydans DSM 2932T, and Acidiferrobacter spp. SP3/III have been isolated and made publically available. Using 16S rRNA sequence data publically available for the Acidiferrobacteraceae, we herein shed light into the molecular taxonomy of this family. Results obtained support the presence of three clades Acidiferrobacter, Sulfuricaulis and Sulfurifustis. Genomic analyses of the genome sequences of A. thiooxydansT and Acidiferrobacter spp. SP3/III indicate that ANI relatedness between the SPIII/3 strain and A. thiooxydansT is below 95–96%, supporting the classification of strain SP3/III as a new species within this genus. In addition, approximately 70% of Acidiferrobacter sp. SPIII/3 predicted genes have a conserved ortholog in A. thiooxydans strains. A comparative analysis of iron, sulfur oxidation pathways, genome plasticity and cell-cell communication mechanisms of Acidiferrobacter spp. are also discussed. © 2018 The AuthorsÍtem Occurrence, integrity and functionality of AcaML1–like viruses infecting extreme acidophiles of the Acidithiobacillus species complex(Elsevier Masson SAS, 2022-12) Covarrubias, Paulo C.; Moya-Beltrán, Ana; Atavales, Joaquín; Moya-Flores, Francisco; Tapia, Pablo S.; Acuña, Lillian G.; Spinelli, Silvia; Quatrini, RaquelGeneral knowledge on the diversity and biology of microbial viruses infecting bacterial hosts from extreme acidic environments lags behind most other econiches. In this study, we analyse the AcaML1 virus occurrence in the taxon, its genetic composition and infective behaviour under standard acidic and SOS-inducing conditions to assess its integrity and functionality. Occurrence analysis in sequenced acidithiobacilli showed that AcaML1-like proviruses are confined to the mesothermophiles Acidithiobacillus caldus and Thermithiobacillus tepidarius. Among A. caldus strains and isolates this provirus had a modest prevalence (30%). Comparative genomic analysis revealed a significant conservation with the T. tepidarius AcaML1-like provirus, excepting the tail genes, and a high conservation of the virus across strains of the A. caldus species. Such conservation extends from the modules architecture to the gene level, suggesting that organization and composition of these viruses are preserved for functional reasons. Accordingly, the AcaML1 proviruses were demonstrated to excise from their host genomes under DNA-damaging conditions triggering the SOS-response and to produce DNA-containing VLPs. Despite this fact, under the conditions evaluated (acidic) the VLPs obtained from A. caldus ATCC 51756 could not produce productive infections of a candidate sensitive strain (#6) nor trigger it lysis. © 2018 The AuthorsÍtem The type IV secretion system of ICEAfe1: Formation of a conjugative pilus in Acidithiobacillus ferrooxidans(Frontiers in Microbiology, 2019) Flores-Ríos, Rodrigo; Moya-Beltrán, Ana; Pareja-Barrueto, Claudia; Arenas-Salinas, Mauricio; Valenzuela, Sebastián; Orellana, Omar; Quatrini, RaquelThe dispersal of mobile genetic elements and their gene cargo relies on type IV secretion systems (T4SS). In this work the ICEAfe1 Tra-type T4SS nanomachine, encoded in the publicly available genome of Acidithiobacillus ferrooxidans ATCC 23270TY, was characterized in terms of its organization, conservation, expression and mating bridge formation. Twenty-one conjugative genes grouped in four genetic clusters encode the ICEAfe1 T4SS, containing all the indispensable functions for the formation and stabilization of the pili and for DNA processing. The clusters' organization resembles that of other mobile genetic elements (such as plasmids and integrative and conjugative elements-ICEs). Sequence conservation, genetic organization and distribution of the tra system in the genomes of other sequenced Acidithiobacillus spp. suggests that the ICEAfe1 T4SS could mediate the lateral gene transfer between related bacteria. All ICEAfe1 T4SS genes are transcriptionally active and expressed from four independent operons. The transcriptional levels of selected marker genes increase in response to Mitomycin C treatment, a DNA damage elicitor that has acknowledged stimulatory effects on excision rates and gene expression of other ICEs, including ICEAfe1. Using a tailor-made pilin-antiserum against ICEAfe1 T4SS TraA pilin and epifluorescence microscopy, the presence of the conjugative pili on the cell surface of A. ferrooxidans could be demonstrated. Additionally, immunodetection assays, by immunogold, allowed the identification of pili-like extracellular structures. Together, the results obtained in this work demonstrate that the ICEAfe1 T4SS is phylogenetically conserved within the taxon, is expressed at mRNA and protein levels in vivo in the A. ferrooxidans type strain, and produces a pili-like structure of extracellular and intercellular localization in this model acidophile, supporting its functionality. Additional efforts will be required to prove conjugation of the ICEAfe1 or parts of this element through the cognate T4SS.