Examinando por Autor "Olmedo, Patricio"

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    Metabolite Profiling Reveals the Effect of Cold Storage on Primary Metabolism in Nectarine Varieties with Contrasting Mealiness
    (MDPI, 2023-02) Olmedo, Patricio; Zepeda, Baltasar; Delgado-Rioseco, Joaquín; Leiva, Carol; Moreno, Adrián A.; Sagredo, Karen; Blanco-Herrera, Francisca; Pedreschi, Romina; Infante, Rodrigo; Meneses, Claudio; Campos-Vargas, Reinaldo
    Chilling injury is a physiological disorder caused by cold storage in peaches and nectarines. The main symptom of chilling injury is mealiness/wooliness, described as a lack of juice in fruit flesh. In this work, we studied two nectarine varieties (Andes Nec-2 and Andes Nec-3) with contrasting susceptibility to mealiness after cold storage. A non-targeted metabolomic analysis was conducted by GC-MS to understand if changes in metabolite abundance are associated with nectarine mealiness induced by cold storage. Multivariate analyses indicated that in unripe nectarines, cold storage promoted a higher accumulation of amino acids in both varieties. Interestingly, for ripe nectarines, cold storage induced an accumulation of fewer amino acids in both varieties and showed an increased abundance of sugars and organic acids. A pathway reconstruction of primary metabolism revealed that in ripe nectarines, cold storage disrupted metabolite abundance in sugar metabolism and the TCA cycle, leading to a differential accumulation of amino acids, organic acids, and sugars in mealy and juicy nectarines. © 2023 by the authors
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    Pre-anthesis cytokinin applications increase table grape berry firmness by modulating cell wall polysaccharides
    (MDPI, 2021-12) Rojas, Bárbara; Suárez-Vega, Felipe; Saez-Aguayo, Susana; Olmedo, Patricio; Zepeda, Baltasar; Delgado-Rioseco, Joaquín; Defilippi, Bruno G.; Pedreschi, Romina; Meneses, Claudio; Pérez-Donoso, Alonso G.; Campos-Vargas, Reinaldo
    The use of plant growth regulators (PGRs) is widespread in commercial table grape vine-yards. The synthetic cytokinin CPPU is a PGR that is extensively used to obtain higher quality grapes. However, the effect of CPPU on berry firmness is not clear. The current study investigated the effects of pre-anthesis applications (BBCH15 and BBCH55 stages) of CPPU on ‘Thompson Seedless’ berry firmness at harvest through a combination of cytological, morphological, and biochemical analyses. Ovaries in CPPU-treated plants presented morphological changes related to cell division and cell wall modification at the anthesis stage (BBCH65). Moreover, immunofluorescence analysis with monoclonal antibodies 2F4 and LM15 against pectin and xyloglucan demonstrated that CPPU treatment resulted in cell wall modifications at anthesis. These early changes have major repercussions regarding the hemicellulose and pectin cell wall composition of mature fruits, and are associated with increased calcium content and a higher berry firmness at harvest. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.