Examinando por Autor "Peppino Margutti, Micaela"

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    Molecular and Genomic Characterization of the Pseudomonas syringae Phylogroup 4: An Emerging Pathogen of Arabidopsis thaliana and Nicotiana benthamiana
    (MDPI, 2022-03) Zavala, Diego; Fuenzalida, Isabel; Gangas, María Victoria; Peppino Margutti, Micaela; Bartoli, Claudia; Roux, Fabrice; Meneses, Claudio; Herrera Vásquez, Ariel; Blanco Herrera, Francisca
    Environmental fluctuations such as increased temperature, water availability, and air CO2 concentration triggered by climate change influence plant disease dynamics by affecting hosts, pathogens, and their interactions. Here, we describe a newly discovered Pseudomonas syringae strain found in a natural population of Arabidopsis thaliana collected from the southwest of France. This strain, called Psy RAYR-BL, is highly virulent on natural Arabidopsis accessions, Arabidopsis model accession Columbia 0, and tobacco plants. Despite the severe disease phenotype caused by the Psy RAYR-BL strain, we identified a reduced repertoire of putative Type III virulence effectors by genomic sequencing compared to P. syringae pv tomato (Pst) DC3000. Furthermore, hopBJ1Psy is found exclusively on the Psy RAYR-BL genome but not in the Pst DC3000 genome. The plant expression of HopBJ1Psy induces ROS accumulation and cell death. In addition, HopBJ1Psy participates as a virulence factor in this plant-pathogen interaction, likely explaining the severity of the disease symptoms. This research describes the characterization of a newly discovered plant pathogen strain and possible virulence mechanisms underlying the infection process shaped by natural and changing environmental conditions. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
  • Miniatura
    Ítem
    Molecular and Genomic Characterization of the Pseudomonas syringae Phylogroup 4: An Emerging Pathogen of Arabidopsis thaliana and Nicotiana benthamiana
    (MDPI, 2022-03) Zavala, Diego; Fuenzalida, Isabel; Gangas, María Victoria; Peppino Margutti, Micaela; Bartoli, Claudia; Roux, Fabrice; Meneses, Claudio; Herrera Vásquez, Ariel; Blanco Herrera, Francisca
    Environmental fluctuations such as increased temperature, water availability, and air CO2 concentration triggered by climate change influence plant disease dynamics by affecting hosts, pathogens, and their interactions. Here, we describe a newly discovered Pseudomonas syringae strain found in a natural population of Arabidopsis thaliana collected from the southwest of France. This strain, called Psy RAYR-BL, is highly virulent on natural Arabidopsis accessions, Arabidopsis model accession Columbia 0, and tobacco plants. Despite the severe disease phenotype caused by the Psy RAYR-BL strain, we identified a reduced repertoire of putative Type III virulence effectors by genomic sequencing compared to P. syringae pv tomato (Pst) DC3000. Furthermore, hopBJ1Psy is found exclusively on the Psy RAYR-BL genome but not in the Pst DC3000 genome. The plant expression of HopBJ1Psy induces ROS accumulation and cell death. In addition, HopBJ1Psy participates as a virulence factor in this plant-pathogen interaction, likely explaining the severity of the disease symptoms. This research describes the characterization of a newly discovered plant pathogen strain and possible virulence mechanisms underlying the infection process shaped by natural and changing environmental conditions. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
  • Miniatura
    Ítem
    Molecular and Genomic Characterization of the Pseudomonas syringae Phylogroup 4: An Emerging Pathogen of Arabidopsis thaliana and Nicotiana benthamiana
    (MDPI, 2022-03) Zavala, Diego; Fuenzalida, Isabel; Gangas, María Victoria; Peppino Margutti, Micaela; Bartoli, Claudia; Roux, Fabrice; Meneses, Claudio; Herrera Vásquez, Ariel; Blanco Herrera, Francisca
    Environmental fluctuations such as increased temperature, water availability, and air CO2 concentration triggered by climate change influence plant disease dynamics by affecting hosts, pathogens, and their interactions. Here, we describe a newly discovered Pseudomonas syringae strain found in a natural population of Arabidopsis thaliana collected from the southwest of France. This strain, called Psy RAYR-BL, is highly virulent on natural Arabidopsis accessions, Arabidopsis model accession Columbia 0, and tobacco plants. Despite the severe disease phenotype caused by the Psy RAYR-BL strain, we identified a reduced repertoire of putative Type III virulence effectors by genomic sequencing compared to P. syringae pv tomato (Pst) DC3000. Furthermore, hopBJ1Psy is found exclusively on the Psy RAYR-BL genome but not in the Pst DC3000 genome. The plant expression of HopBJ1Psy induces ROS accumulation and cell death. In addition, HopBJ1Psy participates as a virulence factor in this plant-pathogen interaction, likely explaining the severity of the disease symptoms. This research describes the characterization of a newly discovered plant pathogen strain and possible virulence mechanisms underlying the infection process shaped by natural and changing environmental conditions. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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    Ítem
    Rhamnolipid-Stabilized Essential Oils Nanoemulsions: Sustainable Biopesticides and Biostimulants with Potential for Crop Protection
    (Multidisciplinary Digital Publishing Institute (MDPI), 0025) Kourdova, Lucille T.; Mottola, Milagro; Peppino Margutti, Micaela; Bogino, María Florencia; Maritano, Paula; Vico, Raquel Viviana; Blanco-Herrera, Francisca; Fanani, María Laura; Fabro, Georgina
    The search for environmentally friendly solutions to effectively control crop pests while safeguarding human health has become a global priority. One promising strategy is to enhance plant defenses by pre-inducing their innate immune system. In this study, we developed rhamnolipid (RL)-stabilized nanoemulsions (NEs) encapsulating essential oils (EOs) as potential biopesticides and biostimulants for agroindustrial applications. These NEs were designed to improve the solubility and stability of EOs while effectively combining their insecticidal and/or repellent activities with the bioactive properties of RLs. In this regard, our interdisciplinary approach involved formulating and characterizing these NEs and evaluating their stability and wettability on plant leaf surfaces. We further evaluated their effects on bacterial growth in vitro and in the model plant Arabidopsis thaliana, along with their impact on beneficial soil microorganisms. We analyzed their ability to stimulate the plant’s immune system and their impact on the viability and reproduction of the aphid Myzus persicae. Additionally, we explored whether RLs stimulate plant defenses through alterations in the leaf cuticle. Our findings demonstrate that RL-stabilized EO-NEs are effective bioprotectants and biostimulants in the model plant, offering a sustainable alternative that could reduce reliance on chemical pesticides in agriculture. © 2025 by the authors.