Examinando por Autor "Plaza Garrido, Angela Valeria"

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    Characterization of ruminococcus sp.spore-like structures
    (Universidad Andrés Bello, 2020) Plaza Garrido, Angela Valeria; Paredes-Sabja, Daniel
    The intestinal microbiota community has a wide diversity of commensal bacteria, whose 50-60% of the bacterial genus contain spore-forming species. Ruminococcus spp., is one of the most abundant species in the human gut-microbiota. It makes to Ruminococcuscrucial for the degradation and fermentation of polysaccharides like cellobiose and starch. Currently, most of Ruminococcusstudies have focus on thesedegradation processes; however, still exist a deep unknowing on this bacteria’s transmission mechanism on humans. Despite that the literature reveals that Ruminococcuscan develop spores, the sporulation process and its properties have not been characterized yet; therefore, this research address the Ruminococcussp.DSM 102803 sporulation process, analyzing its sporulation-dynamic, morphotypes, stability, spores’ germination and ultra-structure protein composition.Results showed a late initiation of Ruminococcusspores compared to Bacillusand Clostridium with presence of four different Ruminococcusmorphotypes during the process: i) phase-dark structure (vegetative cells), ii) division cell, iii) phase-gray structure (intermediary), and iv) phase-bright structure (spore). According to TEM observations, phase-bright structure presents an ultra-structure different than canonical spores; however, it was not possible to see the exosporium, coat, and cortex. Additionally, results indicated that phase-bright structures are structurally stable to lysozyme, ethanol, and heat. At the same time, results reveled that phase-bright structure R.sp.DSM 102803 only are able of initiates germination in presences of sodium taurocholate, some amino acids, and salts; however, these componentsare not sufficient to carry out the process in a canonical way as in other spore-forming species. Finally, the protein analysis concluded that only Spo0J, McsA, Jag, dapG, SpoVS, SpoVK, SpoVAD, SpoVD, and alr2 proteins are specific to one sporulation stage, while no proteins participate on the germination process. These results conclude that the bright phase structures formed by R.sp.DSM 102803 are similar to spores of other spore-forming species. However, the sporulation and germination process must be characterized in greater depth in order to understand the genes and different processes that are involved in germination and sporulation.