Examinando por Autor "Riveros, A."
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Ítem Dormant but Active: Chilling Accumulation Modulates the Epigenome and Transcriptome of Prunus avium During Bud Dormancy(Frontiers Media S.A., 2020-07) Rothkegel, K.; Sandoval, P.; Soto, E.; Ulloa, L.; Riveros, A.; Lillo-Carmona, V.; Cáceres-Molina, J.; Almeida, A.M.; Meneses, C.Temperate deciduous fruit tree species like sweet cherry (Prunus avium) require long periods of low temperatures to trigger dormancy release and flowering. In addition to sequence-based genetic diversity, epigenetic variation may contribute to different chilling requirements among varieties. For the low chill variety ‘Royal Dawn’ and high chill variety ‘Kordia’, we studied the methylome of floral buds during chilling accumulation using MethylC-seq to identify differentially methylated regions (DMRs) during chilling hours (CH) accumulation, followed by transcriptome analysis to correlate changes in gene expression with DNA methylation. We found that during chilling accumulation, DNA methylation increased from 173 CH in ‘Royal Dawn’ and 443 CH in ‘Kordia’ and was mostly associated with the CHH context. In addition, transcriptional changes were observed from 443 CH in ‘Kordia’ with 1,210 differentially expressed genes, increasing to 4,292 genes at 1,295 CH. While ‘Royal Dawn’ showed approximately 5,000 genes differentially expressed at 348 CH and 516 CH, showing a reprogramming that was specific for each genotype. From conserved upregulated genes that overlapped with hypomethylated regions and downregulated genes that overlapped with hypermethylated regions in both varieties, we identified genes related to cold-sensing, cold-signaling, oxidation-reduction process, metabolism of phenylpropanoids and lipids, and a MADS-box SVP-like gene. As a complementary analysis, we used conserved and non-conserved DEGs that presented a negative correlation between DNA methylations and mRNA levels across all chilling conditions, obtaining Gene Ontology (GO) categories related to abiotic stress, metabolism, and oxidative stress. Altogether, this data indicates that changes in DNA methylation precedes transcript changes and may occur as an early response to low temperatures to increase the cold tolerance in the endodormancy period, contributing with the first methylome information about the effect of environmental cues over two different genotypes of sweet cherry. © Copyright © 2020 Rothkegel, Sandoval, Soto, Ulloa, Riveros, Lillo-Carmona, Cáceres-Molina, Almeida and Meneses.Ítem Estudio Anatómico del Fascículo Accesorio del Músculo Flexor Largo del Pulgar y su Relación con el Nervio Interóseo Anterior en Individuos Brasileños(Sociedad Chilena de Anatomía, 2015-03) Riveros, A.; Olave, E.; Sousa-Rodrigues, C.RESUMEN: Entre los síndromes de compresión nerviosa en el antebrazo, debido a la presencia de arcos tendinosos de músculos relacionados al trayecto de los nervios que transcurren entre sus capas musculares, se encuentra el del nervio interóseo anterior (NIA). La presencia de variaciones musculares puede generar arcos que ocasionan compresión nerviosa. El objetivo fue establecer la incidencia y morfología del músculo accesorio del flexor largo del pulgar (AFLP) y la relación de éste con el NIA o sus ramos motores. Se utilizaron 30 antebrazos, de cadáveres formolizados de individuos adultos brasileños. El punto de referencia seleccionado para las mediciones fue el epicóndilo medial del humero (EMH). Se observó que tres miembros superiores presentaron el AFLP (10%), en todos ellos se originó en el EMH para terminar insertándose en el tendón del músculo flexor largo del pulgar (FLP). El vientre muscular presentó aspecto fusiforme, el cual tenía un tendón que formó un arco con el FLP. En uno de los casos, este arco se relacionó con el paso del NIA; en los otros dos, lo hizo con los ramos motores que se originan del NIA. Al diagnosticar los síndromes generados por compresión nerviosa se debe considerar la presencia del AFLP, el cual, potencialmente se puede relacionar con el trayecto del NIA o de sus ramos.Ítem Exercise-induced hypoalgesia in chronic neck pain: A narrative review(Elsevier, 2024-03) Rojas-Galleguillos, F.; Clark-Hormazábal, C.; Méndez-Fuentes, E.; Guede-Rojas, F.; Mendoza, C.; Riveros, A.; Carvajal-Parodi, C.Chronic neck pain (CNP) is a worldwide health problem with several risk factors. One of the most widely used treatments for managing this condition is therapeutic exercise, which could generate a response called exercise-induced hypoalgesia (EIH). There is no consensus on the best exercise modality to induce hypoalgesia. Therefore, this review aims to analyze and synthesize the state-of-the-art about the hypoalgesic effect of exercise in subjects with CNP. We included articles on EIH and CNP in patients older than 18 years, with pain for more than three months, where the EIH response was measured. Articles that studied CNP associated with comorbidities or measured the response to treatments other than exercise were excluded. The studies reviewed reported variable results. Exercise in healthy subjects has been shown to reduce indicators of pain sensitivity; however, in people with chronic pain, the response is variable. Some investigations reported adverse effects with increased pain intensity and decreased pain sensitivity, others found no clinical response, and some even reported EIH with decreased pain and increased sensitivity. EIH is an identifiable, stimulable, and helpful therapeutic response in people with pain. More research is still needed on subjects with CNP to clarify the protocols and therapeutic variables that facilitate the EIH phenomenon. In addition, it is necessary to deepen the knowledge of the intrinsic and extrinsic factors that influence EIH in people with CNP.Ítem Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide(Dove Medical Press Ltd., 2020) Riveros, A.; Eggeling, C.; Riquelme, S.; Adura, C.; López-Iglesias, C.; Guzmán, F.; Araya, E.; Almada, M.; Juárez, J.; Valdez, M.; Fuentevilla, I.; López, O.Gold nanorods are highly reactive, have a large surface-to-volume ratio, and can be functionalized with biomolecules. Gold nanorods can absorb infrared electromagnetic radiation, which is subsequently dispersed as local heat. Gold nanoparticles can be used as powerful tools for the diagnosis and therapy of different diseases. To improve the biological barrier permeation of nanoparticles with low cytotoxicity, in this study, we conjugated gold nanorods with cell-penetrating peptides (oligoarginines) and with the amphipathic peptide CLPFFD. Methods: We studied the interaction of the functionalized gold nanorods with biological membrane models (liposomes) by dynamic light scattering, transmission electron microscopy and the Langmuir balance. Furthermore, we evaluated the effects on cell viability and permeability with an MTS assay and TEM. Results and Discussion: The interaction study by DLS, the Langmuir balance and cryo-TEM support that GNR-Arg7 CLPFFD enhances the interactions between GNRs and biological membranes. In addition, cells treated with GNR-Arg7 CLPFFD internalized 80% more nanoparticles than cells treated with GNR alone and did not induce cell damage. Conclusion: Our results indicate that incorporation of an amphipathic sequence into oligoarginines for the functionalization of gold nanorods enhances biological membrane nanoparticle interactions and nanoparticle cell permeability with respect to nanorods functionalized with oligoarginine. Overall, functionalized gold nanorods with amphipathic arginine rich peptides might be candidates for improving drug delivery by facilitating biological barrier permeation.Ítem Transcriptome analysis of Echinococcus granulosus sensu stricto protoscoleces reveals differences in immune modulation gene expression between cysts found in cattle and sheep(2022-01) Pereira, I.; Hidalgo, C.; Stoore, C.; Baquedano, M.; Cabezas, C.; Bastías, M.; Riveros, A.; Meneses, C.; Cancela, M.; Ferreira, H.; Sáenz, L.; Paredes, R.Cystic Echinococcosis (CE), a zoonotic parasitic disease, is caused by the cestode Echinococcus granulosus sensu lato. CE inflicts severe damage in cattle, sheep, and human hosts worldwide. Fertile CE cysts are characterized by the presence of viable protoscoleces. These parasite forms are studied with minimal contamination with host molecules. Hosts, cattle and sheep, show differences in their CE cyst fertility. The effect of the host in protoscolex transcriptome is not known. We genotyped and performed transcriptomic analysis on sheep protoscoleces obtained from liver and lung CE cysts. The transcriptomic data of Echinococcus granulosus sensu stricto protoscoleces from 6 lung CE cysts and 6 liver CE cysts were Collected. For host comparison analysis, 4 raw data files belonging to Echinococcus granulosus sensu stricto protoscoleces from cattle liver CE cysts were obtained from the NCBI SRA database. Principal component and differential expression analysis did not reveal any statistical differences between protoscoleces obtained from liver or lung cysts, either within the same sheep or different sheep hosts. Conversely, there are significant differences between cattle and sheep protoscolex samples. We found differential expression of immune-related genes. In cattle, 7 genes were upregulated in protoscoleces from liver cysts. In sheep, 3 genes were upregulated in protoscoleces from liver and lung CE cysts. Noteworthy, are the differential expression of antigen B, tegument antigen, and arginase-2 in samples obtained from sheep CE cysts, and basigin in samples from cattle CE cysts. These findings suggest that the host species is an important factor involved in the differential expression of immune related genes, which in turn is possibly related to the fertility of Echinococcus granulosus sensu stricto cysts.Ítem Variations of the Cerebral Arterial Circle. Morphological and Clinical Analysis(Universidad de la Frontera, 2022-06) Riveros, A.; Vega, C.; Negroni, M.; Villagrán, F.; Binvignat, O.; Olave, E.The variations knowledge of the cerebral arterial circle (CAC) is relevant due to its influence on the development of ischemic encephalic disorders. Among these variations, when the external diameter of the posterior communicating artery exceeds the posterior cerebral artery caliber, we have a fetal conformation of this circle. The aim of this study was to describe the variations of the CAC in Chilean individuals and to know the type of arterial conformation. Thirty adult brains were used to measure lengths and caliber of the pre-communicating segments of the anterior (A1) and posterior (P1) cerebral arteries, and the anterior (ACoA) and posterior (PCoA) communicating arteries. The arterial conformation type was established, and the length and caliber of these vessels were compared according to the right or left side. It was observed that 76.6 % of the CACs presented aplasia and / or hypoplasia. Of its components, PCoA was hypoplasic in 53.3 %, appearing bilaterally in 40 % of the subjects. The comparison according to the side, indicated that the mean length of A1 and PCoA on the right side was slightly higher. In the case of caliber, the mean of A1, P1 and PCoA was higher on the left side. Regarding P1 and PcoA caliber, 33 % of the CACs presented unilateral fetal conformation. Regardless of the variability presented by the CAC, there is consensus that PCoA exhibits the greatest variability. The understanding of this variability requires an analysis of the embryonic aspects that can explain the fetal conformation of the CAC in the adult. © 2022, Universidad de la Frontera. All rights reserved.