Examinando por Autor "Schmidt, B."
Mostrando 1 - 4 de 4
Resultados por página
Opciones de ordenación
Ítem Oxidation of isodrimeninol with PCC yields drimane derivatives with activity against candida yeast by inhibition of lanosterol 14-alpha demethylase(MDPI AG, 2020-08) Marin, V.; Iturra, A.; Opazo, A.; Schmidt, B.; Heydenreich, M.; Ortiz, L.; Jiménez, V.A.; Paz, C.Candida species cause an opportunistic yeast infection called Candidiasis, which is responsible for more than 50,000 deaths every year around the world. Effective treatments against candidiasis caused by non-albicans Candida species such as C. glabrata, C. parapsilosis, C. aureus, and C. krusei are limited due to severe resistance to conventional antifungal drugs. Natural drimane sesquiterpenoids have shown promising antifungal properties against Candida yeast and have emerged as valuable candidates for developing new candidiasis therapies. In this work, we isolated isodrimeninol (C1) from barks of Drimys winteri and used it as starting material for the hemi-synthesis of four sesquiterpenoids by oxidation with pyridinium chlorochromate (PCC). The structure of the products (C2, C3, C4, and C5) was elucidated by 1D and 2D NMR spectroscopy resulting in C4 being a novel compound. Antifungal activity assays against C. albicans, C. glabrata, and C. krusei revealed that C4 exhibited an increased activity (IC50 of 75 µg/mL) compared to C1 (IC50 of 125 µg/mL) in all yeast strains. The antifungal activity of C1 and C4 was rationalized in terms of their capability to inhibit lanosterol 14-alpha demethylase using molecular docking, molecular dynamics simulations, and MM/GBSA binding free energy calculations. In silico analysis revealed that C1 and C4 bind to the outermost region of the catalytic site of 14-alpha demethylase and block the entrance of lanosterol (LAN) to the catalytic pocket. Binding free energy estimates suggested that C4 forms a more stable complex with the enzyme than C1, in agreement with the experimental evidence. Based on this new approach it is possible to design new drimane-type sesquiterpenoids for the control of Candida species as inhibitors of 14-alpha demethylase. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.Ítem PESSTO: Survey description and products from the first data release by the Public ESO Spectroscopic Survey of Transient Objects(EDP Sciences, 2015-07) Smartt, S.J.; Valenti, S.; Fraser, M.; Inserra, C.; Young, D.R.; Sullivan, M.; Pastorello, A.; Benetti, S.; Gal-Yam, A.; Knapic, C.; Molinaro, M.; Smareglia, R.; Smith, K.W.; Taubenberger, S.; Yaron, O.; Anderson, J.P.; Ashall, C.; Balland, C.; Baltay, C.; Barbarino, C.; Bauer, F.E.; Baumont, S.; Bersier, D.; Blagorodnova, N.; Bongard, S.; Botticella, M.T.; Bufano, F.; Bulla, M.; Cappellaro, E.; Campbell, H.; Cellier-Holzem, F.; Chen, T.-W.; Childress, M.J.; Clocchiatti, A.; Contreras, C.; Dall'Ora, M.; Danziger, J.; De Jaeger, T.; De Cia, A.; Della Valle, M.; Dennefeld, M.; Elias-Rosa, N.; Elman, N.; Feindt, U.; Fleury, M.; Gall, E.; Gonzalez-Gaitan, S.; Galbany, L.; Morales Garoffolo, A.; Greggio, L.; Guillou, L.L.; Hachinger, S.; Hadjiyska, E.; Hage, P.E.; Hillebrandt, W.; Hodgkin, S.; Hsiao, E.Y.; James, P.A.; Jerkstrand, A.; Kangas, T.; Kankare, E.; Kotak, R.; Kromer, M.; Kuncarayakti, H.; Leloudas, G.; Lundqvist, P.; Lyman, J.D.; Hook, I.M.; Maguire, K.; Manulis, I.; Margheim, S.J.; Mattila, S.; Maund, J.R.; Mazzali, P.A.; McCrum, M.; McKinnon, R.; Moreno-Raya, M.E.; Nicholl, M.; Nugent, P.; Pain, R.; Pignata, G.; Phillips, M.M.; Polshaw, J.; Pumo, M.; Rabinowitz, D.; Reilly, E.; Romero-Cañizales, C.; Scalzo, R.; Schmidt, B.; Schulze, S.; Sim, S.; Sollerman, J.; Taddia, F.; Tartaglia, L.; Terreran, G.; Tomasella, L.; Turatto, M.; Walker, E.; Walton, N.A.; Wyrzykowski, L.; Yuan, F.; Zampieri, L.Context. The Public European Southern Observatory Spectroscopic Survey of Transient Objects (PESSTO) began as a public spectroscopic survey in April 2012. PESSTO classifies transients from publicly available sources and wide-field surveys, and selects science targets for detailed spectroscopic and photometric follow-up. PESSTO runs for nine months of the year, January - April and August - December inclusive, and typically has allocations of 10 nights per month. Aims. We describe the data reduction strategy and data products that are publicly available through the ESO archive as the Spectroscopic Survey data release 1 (SSDR1). Methods. PESSTO uses the New Technology Telescope with the instruments EFOSC2 and SOFI to provide optical and NIR spectroscopy and imaging. We target supernovae and optical transients brighter than 20.5m for classification. Science targets are selected for follow-up based on the PESSTO science goal of extending knowledge of the extremes of the supernova population. We use standard EFOSC2 set-ups providing spectra with resolutions of 13-18 Å between 3345-9995 Å. A subset of the brighter science targets are selected for SOFI spectroscopy with the blue and red grisms (0.935-2.53 μm and resolutions 23-33 Å) and imaging with broadband JHKs filters. Results. This first data release (SSDR1) contains flux calibrated spectra from the first year (April 2012-2013). A total of 221 confirmed supernovae were classified, and we released calibrated optical spectra and classifications publicly within 24 h of the data being taken (via WISeREP). The data in SSDR1 replace those released spectra. They have more reliable and quantifiable flux calibrations, correction for telluric absorption, and are made available in standard ESO Phase 3 formats. We estimate the absolute accuracy of the flux calibrations for EFOSC2 across the whole survey in SSDR1 to be typically ∼15%, although a number of spectra will have less reliable absolute flux calibration because of weather and slit losses. Acquisition images for each spectrum are available which, in principle, can allow the user to refine the absolute flux calibration. The standard NIR reduction process does not produce high accuracy absolute spectrophotometry but synthetic photometry with accompanying JHKs imaging can improve this. Whenever possible, reduced SOFI images are provided to allow this. Conclusions. Future data releases will focus on improving the automated flux calibration of the data products. The rapid turnaround between discovery and classification and access to reliable pipeline processed data products has allowed early science papers in the first few months of the survey. © ESO, 2015.Ítem SN 2012ec: Mass of the progenitor from PESSTO follow-up of the photospheric phase(Oxford University Press, 2015-04) Barbarino, C.; Dall'Ora, M.; Botticella, M.T.; Della Valle, M.; Zampieri, L.; Maund, J.R.; Pumo, M.L.; Jerkstrand, A.; Benetti, S.; Elias-Rosa, N.; Fraser, M.; Gal-Yam, A.; Hamuy, M.; Inserra, C.; Knapic, C.; LaCluyze, A.P.; Molinaro, M.; Ochner, P.; Pastorello, A.; Pignata, G.; Reichart, D.E.; Ries, C.; Riffeser, A.; Schmidt, B.; Schmidt, M.; Smareglia, R.; Smartt, S.J.; Smith, K.; Sollerman, J.; Sullivan, M.; Tomasella, L.; Turatto, M.; Valenti, S.; Yaron, O.; Young, D.We present the results of a photometric and spectroscopic monitoring campaign of SN 2012ec, which exploded in the spiral galaxy NGC 1084, during the photospheric phase. The photometric light curve exhibits a plateau with luminosity L = 0.9 × 1042 erg s-1 and duration ~90 d, which is somewhat shorter than standard Type II-P supernovae (SNe). We estimate the nickel mass M(56Ni) = 0.040 ± 0.015 M⊙ from the luminosity at the beginning of the radioactive tail of the light curve. The explosion parameters of SN 2012ec were estimated from the comparison of the bolometric light curve and the observed temperature and velocity evolution of the ejecta with predictions from hydrodynamical models.We derived an envelope mass of 12.6 M⊙, an initial progenitor radius of 1.6 × 1013 cm and an explosion energy of 1.2 foe. These estimates agree with an independent study of the progenitor star identified in pre-explosion images, for which an initial mass ofM = 14-22 M⊙ was determined.We have applied the same analysis to two other Type II-P SNe (SNe 2012aw and 2012A), and carried out a comparison with the properties of SN 2012ec derived in this paper.We find a reasonable agreement between the masses of the progenitors obtained from pre-explosion images and masses derived from hydrodynamical models. We estimate the distance to SN 2012ec with the standardized candle method (SCM) and compare it with other estimates based on other primary and secondary indicators. SNe 2012A, 2012aw and 2012ec all follow the standard relations for the SCM for the use of Type II-P SNe as distance indicators. © 2015 The Authors.Ítem The new SOXS instrument for the ESO NTT(SPIE, 2016-06) Schipani, P.; Claudi, R.; Campana, S.; Baruffolo, A.; Basa, S.; Basso, S.; Cappellaro, E.; Cascone, E.; Cosentino, R.; D'Alessio, F.; De Caprio, V.; Della Valle, M.a De Ugarte Postigo A.; D'Orsi, S.; Franzen, R.; Fynbo, J.; Gal-Yam, A.; Gardiol, D.; Giro, E.; Hamuy, M.; Iuzzolino, M.; Loreggia, D.; Mattila, S.; Munari, M.; Pignata, G.; Riva, M.; Savarese, S.; Schmidt, B.; Scuderi, S.; Smartt, S.; Vitali, F.SOXS (Son Of X-Shooter) will be a unique spectroscopic facility for the ESO-NTT 3.5-m telescope in La Silla (Chile), able to cover the optical/NIR band (350-1750 nm). The design foresees a high-efficiency spectrograph with a resolutionslit product of ∼4,500, capable of simultaneously observing the complete spectral range 350 - 1750 nm with a good sensitivity, with light imaging capabilities in the visible band. This paper outlines the status of the project. © 2016 SPIE.