Examinando por Autor "Sepulveda, Daniela"

Mostrando 1 - 1 de 1
  • No hay miniatura disponible
    Ítem
    Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule
    (Frontiers Media S.A., 2021-10-25) Ruiz, Pamela; Sepulveda, Daniela; Vidal, José Miguel; Romero, Romina; Contreras, David; Barros, Javier; Carrasco, Carlos; Ruiz-Tagle, Nathaly; Romero, Alex; Urrutia, Homero; Oliver, Cristian
    Piscirickettsia salmonis is the etiological agent of piscirickettsiosis, the most prevalent disease in salmonid species in Chilean salmonids farms. Many bacteria produce N-acyl-homoserine lactones (AHLs) as a quorum-sensing signal molecule to regulate gene expression in a cell density-dependent manner, and thus modulate physiological characteristics and several bacterial mechanisms. In this study, a fluorescent biosensor system method and gas chromatography-tandem mass spectrometry (GC/MS) were combined to detect AHLs produced by P. salmonis. These analyses revealed an emitted fluorescence signal when the biosensor P. putida EL106 (RPL4cep) was co-cultured with both, P. salmonis LF-89 type strain and an EM-90-like strain Ps007, respectively. Furthermore, the production of an AHL-type molecule was confirmed by GC/MS by both P. salmonis strains, which identified the presence of a N-acetyl-L-homoserine Lactone in the supernatant extract. However, It is suggested that an alternate pathway could synthesizes AHLs, which should be address in future experiments in order to elucidate this important bacterial process. To the best of our knowledge, the present report is the first to describe the type of AHLs produced by P. salmonis. Copyright © 2021 Ruiz, Sepulveda, Vidal, Romero, Contreras, Barros, Carrasco, Ruiz-Tagle, Romero, Urrutia and Oliver.