Examinando por Autor "Tapia, Patricio"

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    Identification of grapevine clones via high-throughput amplicon sequencing: a proof-of-concept study
    (Genetics Society of America, 2023-09) Urra, Claudio; Sanhueza, Dayan; Pavez, Catalina; Tapia, Patricio; Núñez-Lillo, Gerardo; Minio, Andrea; Miossec, Matthieu; Blanco-Herrera, Francisca; Gainza, Felipe; Castro, Alvaro; Cantu, Dario; Meneses, Claudio
    Wine cultivars are available to growers in multiple clonal selections with agronomic and enological differences. Phenotypic differences between clones originated from somatic mutations that accrued over thousands of asexual propagation cycles. Genetic diversity between grape cultivars remains unexplored, and tools to discriminate unequivocally clones have been lacking. This study aimed to uncover genetic variations among a group of clonal selections of 4 important Vitis vinifera cultivars: Cabernet sauvignon, Sauvignon blanc, Chardonnay, and Merlot, and use this information to develop genetic markers to discriminate the clones of these cultivars. We sequenced with short-read sequencing technology the genomes of 18 clones, including biological replicates for a total of 46 genomes. Sequences were aligned to their respective cultivar’s reference genome for variant calling. We used reference genomes of Cabernet sauvignon, Chardonnay, and Merlot and developed a de novo genome assembly of Sauvignon blanc using long-read sequencing. On average, 4 million variants were detected for each clone, with 74.2% being single nucleotide variants and 25.8% being small insertions or deletions (InDel). The frequency of these variants was consistent across all clones. From these variants, we validated 46 clonal markers using high-throughput amplicon sequencing for 77.7% of the evaluated clones, most of them small InDel. These results represent an advance in grapevine genotyping strategies and will benefit the viticulture industry for the characterization and identification of the plant material. © The Author(s) 2023.
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    Proyecto periodístico : Elemental Comunicaciones
    (Universidad Andrés Bello, 2007) Tapia, Patricio; Acuña, Víctor.; Moya, Oscar.; Bechan, Deborah; Facultad de Humanidades y Educación; Escuela de Periodismo
    el mundo que vivimos hoy en día, así lo demuestra una serie de ejemplos multimodales que fundamentan su quehacer por medio de las imágenes, es así en Internet, la televisión, periódicos y revistas, en donde el lenguaje visual posee la importancia de generar un primer impacto, el que es previo a cualquier juicio o deducción a posteriori. Ante esta realidad Elemental Comunicaciones es creada con el fin de generar un ente encargado de mejorar la imagen de personas que deseen estar preparadas para enfrentar el mundo, mundo de la imagen y de la instantaneidad comunicacional. Donde cada vez más personas necesitan de la seguridad que brindan el verse bien, hablar bien y comportarse de acuerdo a las circunstancias. Sin duda Elemental Comunicaciones se ubicará como líder de la asesoría de imagen debido a la nutrida oferta de áreas de explotación al mejoramiento del Marketing personal de nuestros clientes, para que con seguridad se enfrenten al mundo actual según las necesidades que nos planteen o que nuestro sólido equipo profesional pueda descubrir. Entre las diferentes opciones con que cuenta Elemental Comunicaciones están el mejoramiento de la imagen estética, e! mejoramiento de las capacidades neutro lingüísticas, enseñanza de estrategias comunicacionales y protocolares, y un cálido equipo humano que no dudara en dar la mejor atención a nuestros clientes,
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    SARS-CoV-2 infection in asymptomatic healthcare workers at a clinic in Chile
    (Public Library of Science, 2021-01) Olmos, Claudio; Campaña, Gonzalo; Monreal, Victor; Pidal, Paola; Sanchez, Nannet; Airola, Constanza; Sanhueza, Dayan; Tapia, Patricio; Muñoz, Ana María; Corvalan, Felipe; Meneses, Claudio; Orellana, Ariel; Montecino, Martin; Arriagada, Gloria; Bustos, Fernando Jose
    Asymptomatic SARS-CoV-2 infection of healthcare workers (HCWs) has been reported as a key player in the nosocomial spreading of COVID-19. Early detection of infected HCWs can prevent spreading of the virus in hospitals among HCWs and patients. We conducted a cross-sectional study to determine the asymptomatic infection of HCWs in a private clinic in the city of Santiago, Chile. Our study was conducted during a period of 5 weeks at the peak of transmission of SARS-CoV-2 in Chile. Nasopharyngeal samples were obtained from 413 HCWs and tested for the presence of SARS-CoV-2 using RT-qPCR. We found that a 3.14% of HCWs were positive for the presence of SARS-CoV-2 (14/413). Out of these, 7/14 were completely asymptomatic and did not develop symptoms within 3 weeks of testing. Sequencing of viral genomes showed the predominance of the GR clade; however, sequence comparison demonstrated numerous genetic differences among them suggesting community infection as the main focus of transmission among HCWs. Our study demonstrates that the protocols applied to protect HCWs and patients have been effective as no infection clusters due to asymptomatic carriers were found in the clinic. Together, these data suggest that infection with SARS-CoV-2 among HCWs of this health center is not nosocomial. © 2021 Olmos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.