Examinando por Autor "Urbina, Daniela"
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Ítem Are EFL teacher implementing the CLL method in 5th and 6th grades within the province of Valparaíso?(Universidad Andrés Bello, 2015) Aguilera, Josefa; Urbina, Daniela; Zapata, Jaime; González Acevedo, Eduardo Adrián; Facultad de Humanidades y Educación; Escuela de Pedagogía en InglésThe purpose of this qualitative investigation is to find out whether Cooperative Language Learning (CLL) is being considered currently as a teaching method, and the frequency of its use, since this method is suggested by the Chilean curriculum. This is a qualitative, exploratory, explicative research. The universe are EFL teachers of the province of Valparaiso and the randomized sample are EFL teachers from 10 different school in that area. The instruments were 30 questionnaires and interviews. The results of research were that the teachers do consider the use of the CLL method, however it was only being used once a month by the majority of them because the large amount of students in the classroom was the main constrain. The conclusion is that EFL teachers do consider the use of the CLL method, notwithstanding this, the frequency of is use could be enhanced if the number of students per classroom was reduced in number.Ítem The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana(Sociedad de Biología de Chile, 2006) Urbina, Daniela; Silva, Herman; Meisel, LeeThapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 µM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropism.