Examinando por Autor "Vahlenkamp, Thomas W."

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    High seroprevalence of feline morbilliviruses in free-roaming domestic cats in Chile
    (Archives of Virology, Volume 166, Issue 1, Pages 281 - 285January 2021, 2021) Busch, Johannes; Sacristán, Irene; Cevidanes, Aitor; Millán, Javier; Vahlenkamp, Thomas W.; Napolitano, Constanza; Napolitano C.; Sieg, Michael
    Feline morbillivirus infections have gained increased attention due to repeated reports of their association with urinary tract disease in cats. In the present study, 112 serum samples from free-roaming domestic cats in Chile were tested for antibodies against feline morbillivirus genotypes 1 and 2 (FeMV-1 and FeMV-2) using an indirect immunofluorescence assay. In total, 63% of the animals showed antibodies against one or both FeMV genotypes. Antibodies directed exclusively against FeMV-2 were significantly more prevalent in male cats. The correlation of sex and FeMV-2 infection might give insight into potential routes of transmission. We provide, for the first time, serological data on FeMV in Chile. © 2020, The Author(s).
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    Identification of novel feline paramyxoviruses in guignas (Leopardus guigna) from Chile
    (MDPI, 2020-12) Sieg, Michael; Sacristán, Irene; Busch, Johannes; Terio, Karen A.; Cabello, Javier; Hidalgo-Hermoso, Ezequiel; Millán, Javier; Böttcher, Denny; Heenemann, Kristin; Vahlenkamp, Thomas W.; Napolitano, Constanza
    The family of paramyxoviruses has received growing attention as several new species have been identified recently, notably two different clusters in domestic cats, designated as feline morbillivirus (FeMV) and feline paramyxovirus (FPaV). Their phylogenetic origin and whether wild felids also harbor these viruses are currently unknown. Kidney samples from 35 guignas (Leopardus guigna), a wild felid from Chile, were investigated for paramyxoviruses using consensus-RT-PCR. In addition, thirteen serum samples of guignas were screened for the presence of FeMV-specific antibodies by an immunofluorescence assay (IFA). Viral RNA was detected in 31% of the kidney samples. Phylogenetic analyses revealed two well-supported clusters, related to isolates from domestic cats, rodents and bats. No significant histopathology changes were recorded in infected guignas. Serology identified two samples which were positive for FeMV-specific antibodies. Our study highlights the diversity of paramyxovirus infections in felids with special emphasis on guignas from Chile.
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    Molecular and serological survey of carnivore pathogens in free-roaming domestic cats of rural communities in southern chile
    (Japanese Society of Veterinary Science, 2019-12) Sacristán, Irene; Sieg, Michael; Acuña, Francisca; Aguilar, Emilio; García, Sebastián; López, María José; Cevidanes, Aitor; Hidalgo-Hermoso, Ezequiel; Cabello, Javier; Vahlenkamp, Thomas W.; Millán, Javier; Poulin, Elie; Napolitano, Constanza
    Owned, free-roaming domestic cats are abundant in the Chilean countryside, having high probability of contact with wildlife and potentially participating as reservoirs of zoonotic pathogens. In the present study, 131 cats from two remote study areas (Valdivia and Chiloe Island) in southern Chile were analyzed for infection/exposure to eight pathogens. Serum samples from 112 cats were tested for antigens against feline leukemia virus (FeLV antigen-ELISA) and antibodies against feline immunodeficiency virus (FIV-ELISA) and canine distemper virus (CDV-serum neutralization), yielded occurrence of 8.9, 1.7 and 0.8% respectively. The presence of DNA of five vector-borne pathogens, piroplasmids, Ehrlichia spp., Anaplasma spp., Rickettsia spp. and Bartonella spp. was investigated in thirty cats. Overall observed occurrence was 6.6% (2/30) for both Anaplasma platys, and B. henselae, and 3.3% (1/30) for both Bartonella sp. and Theileria equi. Observed occurrence for all vector-borne pathogens in Valdivia area was significantly higher than in Chiloe Island (5/15 vs 0/15; P=0.04). Our results represent the first description of exposure to CDV and DNA detection of T. equi and A. platys in domestic cats in Chile. The results highlight the importance of performing pathogen screening in owned, free-roaming rural cats to evaluate their potential role as reservoirs of infection and vectors for disease transmission to wildlife.