Examinando por Autor "Vio, Valentina"

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    Gold nanorods/siRNA complex administration for knockdown of PARP-1: A potential treatment for perinatal asphyxia
    (Dove Medical Press Ltd., 2018) Vio, Valentina; Riveros, Ana L.; Tapia-Bustos, Andrea; Lespay-Rebolledo, Carolyne; Pérez-Lobos, Ronald; Muñoz, Luis; Pismante, Paola; Morales, Paola; Araya, Eyleen; Hassan, Natalia; Herrera-Marschitz, Mario; Kogan, Marcelo J.
    Background: Perinatal asphyxia interferes with neonatal development, resulting in long-term deficits associated with systemic and neurological diseases. Despite the important role of poly (ADP-ribose) polymerase 1 (PARP-1) in the regulation of gene expression and DNA repair, overactivation of PARP-1 in asphyxia-exposed animals worsens the ATP-dependent energetic crisis. Inhibition of PARP-1 offers a therapeutic strategy for diminishing the effects of perinatal asphyxia. Methods: We designed a nanosystem that incorporates a specific siRNA for PARP-1 knockdown. The siRNA was complexed with gold nanorods (AuNR) conjugated to the peptide CLPFFD for brain targeting. Results: The siRNA was efficiently delivered into PC12 cells, resulting in gene silencing. The complex was administered intraperitoneally in vivo to asphyxia-exposed rat pups, and the ability of the AuNR-CLPFFD/siRNA complex to reach the brain was demonstrated. Conclusion: The combination of a nanosystem for delivery and a specific siRNA for gene silencing resulted in effective inhibition of PARP-1 in vivo. © 2018 Vio et al.