Examinando por Autor "Wagemans, Jeroen"

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    ECOPHAGE: Combating Antimicrobial Resistance Using Bacteriophages for Eco-Sustainable Agriculture and Food Systems
    (Multidisciplinary Digital Publishing Institute (MDPI), 2023-11) García, Pilar; Tabla, Rafael; Anany, Hany; Bastias, Roberto; Brøndsted, Lone; Casado, Susana; Cifuentes, Pablo; Deaton, John; Denes, Thomas G.; Islam, Mohammad Aminul; Lavigne, Rob; Moreno-Switt, Andrea I.; Nakayama, Natsuko; Muñoz Madero, Cristina; Sulakvelidze, Alexander; Svircev, Antonet M.; Wagemans, Jeroen; Biosca, Elena G.; Rivera, Dácil
    The focus of this meeting was to discuss the suitability of using bacteriophages as alternative antimicrobials in the agrifood sector. Following a One Health approach, the workshop explored the possibilities of implementing phage application strategies in the agriculture, animal husbandry, aquaculture, and food production sectors. Therefore, the meeting had gathered phage researchers, representatives of the agrifood industry, and policymakers to debate the advantages and potential shortcomings of using bacteriophages as alternatives to traditional antimicrobials and chemical pesticides. Industry delegates showed the latest objectives and demands from consumers. Representatives of regulatory agencies (European Medicines Agency (EMA) and Spanish Agency of Medicines and Health Products (AEMPS)) presented an update of new regulatory aspects that will impact and support the approval and implementation of phage application strategies across the different sectors.
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    Novel Salmonella Phage, vB_Sen_STGO-35-1, Characterization and Evaluation in Chicken Meat
    (MDPI, 2022-03) Rivera, Dácil; Moreno Switt, Andrea I.; Denes, Thomas G.; Hudson, Lauren K.; Peters, Tracey L.; Samir, Reham; Aziz, Ramy K.; Noben, Jean-Paul; Wagemans, Jeroen; Dueñas, Fernando
    Salmonellosis is one of the most frequently reported zoonotic foodborne diseases world-wide, and poultry is the most important reservoir of Salmonella enterica serovar Enteritidis. The use of lytic bacteriophages (phages) to reduce foodborne pathogens has emerged as a promising bio-control intervention for Salmonella spp. Here, we describe and evaluate the newly isolated Salmonella phage STGO-35-1, including: (i) genomic and phenotypic characterization, (ii) an analysis of the reduction of Salmonella in chicken meat, and (iii) genome plasticity testing. Phage STGO-35-1 repre-sents an unclassified siphovirus, with a length of 47,483 bp, a G + C content of 46.5%, a headful strategy of packaging, and a virulent lifestyle. Phage STGO-35-1 reduced S. Enteritidis counts in chicken meat by 2.5 orders of magnitude at 4 °C. We identified two receptor-binding proteins with affinity to LPS, and their encoding genes showed plasticity during an exposure assay. Phenotypic, proteomic, and genomic characteristics of STGO-35-1, as well as the Salmonella reduction in chicken meat, support the potential use of STGO-35-1 as a targeted biocontrol agent against S. Enteritidis in chicken meat. Additionally, computational analysis and a short exposure time assay allowed us to predict the plasticity of genes encoding putative receptor-binding proteins. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.