Membrane vesicles from Piscirickettsia salmonis induce protective immunity and reduce development of salmonid rickettsial septicemia in an adult zebrafish model

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dc.contributor.authorTandberg, Julia
dc.contributor.authorOliver, Cristian
dc.contributor.authorLagos, Leidy
dc.contributor.authorGaarder, Mona
dc.contributor.authorYáñez, Alejandro J.
dc.contributor.authorRopstad, Erik
dc.contributor.authorWinther-Larsen, Hanne C.
dc.date.accessioned2024-04-25T13:29:45Z
dc.date.available2024-04-25T13:29:45Z
dc.date.issued2017-08
dc.descriptionIndexación: Scopus
dc.description.abstractInfections caused by the facultative intracellular bacterial pathogen Piscirickettsia salmonis remains an unsolved problem for the aquaculture as no efficient treatments have been developed. As a result, substantial amounts of antibiotic have been used to limit salmonid rickettsial septicemia (SRS) disease outbreaks. The antibiotic usage has not reduced the occurrence, but lead to an increase in resistant strains, underlining the need for new treatment strategies. P. salmonis produce membrane vesicles (MVs); small spherical structures know to contain a variety of bacterial components, including proteins, lipopolysaccharides (LPS), DNA and RNA. MVs mimics' in many aspects their mother cell, and has been reported as alternative vaccine candidates. Here, MVs from P. salmonis was isolated and evaluated as a vaccine candidate against SRS in an adult zebrafish infection model. When zebrafish was immunized with MVs they were protected from subsequent challenge with a lethal dose of P. salmonis. Histological analysis showed a reduced bacterial load upon challenge in the MV immunized group, and the mRNA expression levels of several immune related genes altered, including mpeg1.1, tnfα, il1b, il10 and il6. The MVs induced the secretion of IgM upon immunization, indicating an immunogenic effect of the vesicles. Taken together, the data demonstrate a vaccine potential of MVs against P. salmonis. © 2017 The Author(s)
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S1050464817303364?via%3Dihub
dc.identifier.citationFish and Shellfish Immunology Volume 67, Pages 189 - 198August 2017
dc.identifier.doi10.1016/j.fsi.2017.06.015
dc.identifier.issn1050-4648
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/56412
dc.language.isoen
dc.publisherAcademic Press
dc.rights.licenseCC BY 4.0 DEED Atribución 4.0 Internacional
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectImmune response
dc.subjectMVs
dc.subjectPiscirickettsia salmonis
dc.subjectSRS
dc.subjectVaccine
dc.subjectZebrafish
dc.titleMembrane vesicles from Piscirickettsia salmonis induce protective immunity and reduce development of salmonid rickettsial septicemia in an adult zebrafish model
dc.typeArtículo
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