In vitro genomic and proteomic evidence of a type IV pili-like structure in the fish pathogen Piscirickettsia salmonis

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dc.contributor.authorŚanchez, Patricio
dc.contributor.authorOliver, Cristian
dc.contributor.authorHernández, Mauricio
dc.contributor.authorCortés, Marcos
dc.contributor.authorCecilia Rauch, María
dc.contributor.authorValenzuela, Karla
dc.contributor.authorGarduno, Rafael A.
dc.contributor.authorAvendano-Herrera, Rubén
dc.contributor.authorYánez, Alejandro J.
dc.date.accessioned2022-07-26T22:45:23Z
dc.date.available2022-07-26T22:45:23Z
dc.date.issued2018-08
dc.descriptionIndexación Scopuses
dc.description.abstractPiscirickettsia salmonis is an intracellular γ -proteobacteria and the etiological agent of piscirickettsiosis, which causes massive economic losses in the Chilean salmon industry. The type IV pili (T4P) play an important role in adherence to host cell surfaces and bacterial pathogenicity. T4P contains a variable number of components, as predicted in P. salmonis genomes. However, no studies have determined if P. salmonis possesses T4P. The aims of this investigation were to identify T4P components in the P. salmonis type strain LF-89T, evaluate respective transcript expressions, and analyze the main putative T4P proteins using bioinformatics and proteomic approaches. Two main clusters of P. salmonis T4P genes were found. Expression of the pilA gene was upregulated at 4 h post-infection (hpi), while pilQ was upregulated 4 days post-infection. At 16 hpi, pilB and pilD were strongly upregulated. The PilA amino acid sequence analysis showed a conserved N-terminal domain and sequence motifs critical for T4P biosynthesis. MudPIT analysis revealed PilA in the P. salmonis LF-89T proteome, and TEM showed pili-like filamentous structures on the P. salmonis surface. These results strongly suggest the presence of a T4P-like structure in P. salmonis. © FEMS 2018.es
dc.description.urihttps://academic-oup-com.recursosbiblioteca.unab.cl/femsle/article/365/16/fny169/5050058
dc.identifier.citationFEMS Microbiology Letters Volume 365, Issue 161 August 2018 Article number fny169es
dc.identifier.doi10.1093/femsle/fny169
dc.identifier.issn03781097
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/23352
dc.language.isoenes
dc.publisherOxford University Presses
dc.rights.licenseCC BY 4.0
dc.subjectFimbriaees
dc.subjectType II Secretion Systemes
dc.subjectTwitchinges
dc.subjectPiscirickettsia salmonises
dc.subjectPiscirickettsiosises
dc.titleIn vitro genomic and proteomic evidence of a type IV pili-like structure in the fish pathogen Piscirickettsia salmonises
dc.typeArtículoes
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