Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas

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dc.contributor.authorBlanco, Rancés
dc.contributor.authorCarrillo Beltrán, Diego
dc.contributor.authorMuñoz, Juan P.
dc.contributor.authorOsorio, Julio C.
dc.contributor.authorTapia, Julio C.
dc.contributor.authorBurzio, Verónica A.
dc.contributor.authorGallegos, Iván
dc.contributor.authorCalaf, Gloria M.
dc.contributor.authorChabay, Paola
dc.contributor.authorAguayo, Francisco
dc.date.accessioned2022-06-01T20:26:42Z
dc.date.available2022-06-01T20:26:42Z
dc.date.issued2022-04
dc.descriptionIndexación: Scopuses
dc.description.abstractHigh-risk human papillomaviruses (HR-HPVs) are the etiological agents of cervical cancer. However, a low proportion of HR-HPV-infected women finally develop this cancer, which suggests the involvement of additional cofactors. Epstein–Barr virus (EBV) has been detected in cervical squamous cell carcinomas (SCCs) as well as in low-(LSIL) and high-grade (HSIL) squamous in-traepithelial lesions, although its role is unknown. In this study, we characterized HR-HPV/EBV co-presence and viral gene expression in LSIL (n = 22), HSIL (n = 52), and SCC (n = 19) from Chilean women. Additionally, phenotypic changes were evaluated in cervical cancer cells ectopically expressing BamHI-A Rightward Frame 1 (BARF1). BARF1 is a lytic gene also expressed in EBV-positive epithelial tumors during the EBV latency program. HPV was detected in 6/22 (27.3%) LSIL, 38/52 (73.1%) HSIL, and 15/19 (78.9%) SCC cases (p < 0.001). On the other hand, EBV was detected in 16/22 (72.7%) LSIL, 27/52 (51.9%) HSIL, and 13/19 (68.4%) SCC cases (p = 0.177). HR-HPV/EBV co-presence was detected in 3/22 (13.6%) LSIL, 17/52 (32.7%) HSIL, and 11/19 (57.9%) SCC cases (p = 0.020). Additionally, BARF1 transcripts were detected in 37/55 (67.3%) of EBV positive cases and in 19/30 (63.3%) of HR-HPV/EBV positive cases. Increased proliferation, migration, and epithelial-mesenchymal transition (EMT) was observed in cervical cancer cells expressing BARF1. Thus, both EBV and BARF1 transcripts are detected in low-and high-grade cervical lesions as well as in cervical carcinomas. In addition, BARF1 can modulate the tumor behavior in cervical cancer cells, suggesting a role in increasing tumor aggressiveness. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.description.urihttps://www.mdpi.com/2076-2607/10/5/888
dc.identifier.citationMicroorganisms Volume 10, Issue 5May 2022 Article number 888es
dc.identifier.doi10.3390/microorganisms10050888
dc.identifier.issn2076-2607
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/22685
dc.language.isoenes
dc.publisherMDPIes
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectBamHI-A rightward frame 1 expressiones
dc.subjectCervical canceres
dc.subjectEpstein–Barr viruses
dc.subjectHuman papillomaviruses
dc.titleCharacterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomases
dc.typeArtículoes
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