Comparative pan-genome analysis of Piscirickettsia salmonis reveals genomic divergences within genogroups

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dc.contributor.authorNourdin-Galindo, Guillermo
dc.contributor.authorSánchez, Patricio
dc.contributor.authorMolina, Cristian F.
dc.contributor.authorEspinoza-Rojas, Daniela A.
dc.contributor.authorOliver, Cristian
dc.contributor.authorRuiz, Pamela
dc.contributor.authorVargas-Chacoff, Luis
dc.contributor.authorCárcamo, Juan G.
dc.contributor.authorFigueroa, Jaime E.
dc.contributor.authorMancilla, Marcos
dc.contributor.authorMaracaja-Coutinho, Vinicius
dc.contributor.authorYañez, Alejandro J.
dc.date.accessioned2023-11-21T17:05:30Z
dc.date.available2023-11-21T17:05:30Z
dc.date.issued2017-11
dc.descriptionIndexación: Scopuses
dc.description.abstractPiscirickettsia salmonis is the etiological agent of salmonid rickettsial septicemia, a disease that seriously affects the salmonid industry. Despite efforts to genomically characterize P. salmonis, functional information on the life cycle, pathogenesis mechanisms, diagnosis, treatment, and control of this fish pathogen remain lacking. To address this knowledge gap, the present study conducted an in silico pan-genome analysis of 19 P. salmonis strains from distinct geographic locations and genogroups. Results revealed an expected open pan-genome of 3,463 genes and a core-genome of 1,732 genes. Two marked genogroups were identified, as confirmed by phylogenetic and phylogenomic relationships to the LF-89 and EM-90 reference strains, as well as by assessments of genomic structures. Different structural configurations were found for the six identified copies of the ribosomal operon in the P. salmonis genome, indicating translocation throughout the genetic material. Chromosomal divergences in genomic localization and quantity of genetic cassettes were also found for the Dot/Icm type IVB secretion system. To determine divergences between core-genomes, additional pan-genome descriptions were compiled for the so-termed LF and EM genogroups. Open pan-genomes composed of 2,924 and 2,778 genes and core-genomes composed of 2,170 and 2,228 genes were respectively found for the LF and EM genogroups. The core-genomes were functionally annotated using the Gene Ontology, KEGG, and Virulence Factor databases, revealing the presence of several shared groups of genes related to basic function of intracellular survival and bacterial pathogenesis. Additionally, the specific pan-genomes for the LF and EM genogroups were defined, resulting in the identification of 148 and 273 exclusive proteins, respectively. Notably, specific virulence factors linked to adherence, colonization, invasion factors, and endotoxins were established. The obtained data suggest that these genes could be directly associated with inter-genogroup differences in pathogenesis and host-pathogen interactions, information that could be useful in designing novel strategies for diagnosing and controlling P. salmonis infection. © 2017 Nourdin-Galindo, Sánchez, Molina, Espinoza-Rojas, Oliver, Ruiz, Vargas-Chacoff, Cárcamo, Figueroa, Mancilla, Maracaja-Coutinho and Yañez.es
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fcimb.2017.00459/full
dc.identifier.citationFrontiers in Cellular and Infection Microbiology Volume 7, Issue OCT31 October 2017 Article number 459es
dc.identifier.doi10.3389/fcimb.2017.00459
dc.identifier.issn2235-2988
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/54010
dc.language.isoenes
dc.publisherFrontiers Media S.A.es
dc.rights.licenseCC BY 4.0 DEED
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectComparative genomicses
dc.subjectFish pathogenes
dc.subjectGammaproteobacteriaes
dc.subjectPan-genomees
dc.subjectPiscirickettsiosises
dc.subjectSalmonid rickettsial septicemiaes
dc.subjectVirulence factorses
dc.titleComparative pan-genome analysis of Piscirickettsia salmonis reveals genomic divergences within genogroupses
dc.typeArtículoes
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