A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids

Vista sencilla de ítem
dc.contributor.authorSteinberg, X.
dc.contributor.authorGalpin, J.
dc.contributor.authorNasir, G.
dc.contributor.authorSepulveda, R.V.
dc.contributor.authorLadron de Guevara, E.
dc.contributor.authorGonzalez-Nilo, F.
dc.contributor.authorIslas, L.D.
dc.contributor.authorAhern, C.A.
dc.contributor.authorBrauchi, S.E.
dc.date.accessioned2021-07-27T13:34:20Z
dc.date.available2021-07-27T13:34:20Z
dc.date.issued2020-10
dc.descriptionIndexación: Scopus.es
dc.description.abstractThe incorporation of non-canonical amino acids into proteins has emerged as a promising strategy to manipulate and study protein structure-function relationships with superior precision in vitro and in vivo. To date, fluorescent non-canonical amino acids (f-ncAA) have been successfully incorporated in proteins expressed in bacterial systems, Xenopus oocytes, and HEK-293T cells. Here, we describe the rational generation of a novel orthogonal aminoacyl-tRNA synthetase based on the E. coli tyrosine synthetase that is capable of encoding the f-ncAA tyr-coumarin in HEK-293T cells. © 2020Bioorganic Chemistry; Bioinformatics; Proteins; Biochemistry; Molecular Biology; Unnatural amino acids, aminoacyl-tRNA synthetase, coumarin, fluorescence. © 2020es
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S2405844020319836?via%3Dihub
dc.identifier.citationHeliyonOpen AccessVolume 6, Issue 10October 2020 Article number e05140es
dc.identifier.doi10.1016/j.heliyon.2020.e05140
dc.identifier.issn2405-8440
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/19520
dc.language.isoenes
dc.publisherElsevier Ltdes
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAminoacyl-tRNA synthetasees
dc.subjectBiochemistryes
dc.subjectBioinformaticses
dc.subjectBioorganic chemistryes
dc.subjectCoumarines
dc.subjectFluorescencees
dc.subjectMolecular biologyes
dc.subjectProteinses
dc.subjectUnnatural amino acidses
dc.titleA rationally designed orthogonal synthetase for genetically encoded fluorescent amino acidses
dc.typeArtículoes
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Steinberg, X._A rationally.pdf
Tamaño:
1.13 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLÉS
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista