Genomic characterization of salmonella Cerro ST367, an emerging Salmonella subtype in cattle in the United States
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2014-06
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Facultad/escuela
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en
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BioMed Central Ltd.
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Atribution 4.0 International (CC BY 4.0)
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https://creativecommons.org/licenses/by/4.0/deed.es
Resumen
Background: Within the last decade, Salmonella enterica subsp. enterica serovar Cerro (S. Cerro) has become one of
the most common serovars isolated from cattle and dairy farm environments in the northeastern US. The fact that
this serovar is commonly isolated from subclinically infected cattle and is rarely associated with human disease,
despite its frequent isolation from cattle, has led to the hypothesis that this emerging serovar may be characterized
by reduced virulence. We applied comparative and population genomic approaches to (i) characterize the
evolution of this recently emerged serovar and to (ii) gain a better understanding of genomic features that could
explain some of the unique epidemiological features associated with this serovar.
Results: In addition to generating a de novo draft genome for one Salmonella Cerro strain, we also generated
whole genome sequence data for 26 additional S. Cerro isolates, including 16 from cattle operations in New York
(NY) state, 2 from human clinical cases from NY in 2008, and 8 from diverse animal sources (7 from Washington
state and 1 from Florida). All isolates sequenced in this study represent sequence type ST367. Population genomic
analysis showed that isolates from the NY cattle operations form a well-supported clade within S. Cerro ST367
(designated here “NY bovine clade”), distinct from isolates from Washington state, Florida and the human clinical
cases. A molecular clock analysis indicates that the most recent common ancestor of the NY bovine clade dates
back to 1998, supporting the recent emergence of this clone.
Comparative genomic analyses revealed several relevant genomic features of S. Cerro ST367, that may be responsible
for reduced virulence of S. Cerro, including an insertion creating a premature stop codon in sopA. In addition, patterns
of gene deletion in S. Cerro ST367 further support adaptation of this clone to a unique ecological or host related niche.
Conclusions: Our results indicate that the increase in prevalence of S. Cerro ST367 is caused by a highly clonal
subpopulation and that S. Cerro ST367 is characterized by unique genomic deletions that may indicate adaptation to
specific ecological niches and possibly reduced virulence in some hosts
Notas
Indexación: Scopus.
Palabras clave
Multi Locus Sequence Typing, Human Intestinal Epithelial Cell, Gaussian Markov Random Field, High Probability Density, Prophage Region
Citación
BMC Genomics. Volume 15, Issue 1. 4 June 2014. Article number 427
DOI
DOI: 10.1186/1471-2164-15-427