Long Noncoding RNA TALAM1 Is a Transcriptional Target of the RUNX2 Transcription Factor in Lung Adenocarcinoma

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dc.contributor.authorBermúdez, Gisella
dc.contributor.authorBernal, Camila
dc.contributor.authorOtalora, Andrea
dc.contributor.authorSanchez, Paula
dc.contributor.authorNardocci, Gino
dc.contributor.authorCañas, Alejandra
dc.contributor.authorLopez-Kleine, Liliana
dc.contributor.authorMontecino, Martín
dc.contributor.authorRojas, Adriana
dc.date.accessioned2023-10-17T13:05:25Z
dc.date.available2023-10-17T13:05:25Z
dc.date.issued2023-09
dc.descriptionIndexación: Scopus.es
dc.description.abstractBackground: Lung cancer is the leading cause of cancer death worldwide. It has been reported that genetic and epigenetic factors play a crucial role in the onset and evolution of lung cancer. Previous reports have shown that essential transcription factors in embryonic development contribute to this pathology. Runt-related transcription factor (RUNX) proteins belong to a family of master regulators of embryonic developmental programs. Specifically, RUNX2 is the master transcription factor (TF) of osteoblastic differentiation, and it can be involved in pathological conditions such as prostate, thyroid, and lung cancer by regulating apoptosis and mesenchymal–epithelial transition processes. In this paper, we identified TALAM1 (Metastasis Associated Lung Adenocarcinoma Transcript 1) as a genetic target of the RUNX2 TF in lung cancer and then performed functional validation of the main findings. Methods: We performed ChIP-seq analysis of tumor samples from a patient diagnosed with lung adenocarcinoma to evaluate the target genes of the RUNX2 TF. In addition, we performed shRNA-mediated knockdown of RUNX2 in this lung adenocarcinoma cell line to confirm the regulatory role of RUNX2 in TALAM1 expression. Results: We observed RUNX2 overexpression in cell lines and primary cultured lung cancer cells. Interestingly, we found that lncRNA TALAM1 was a target of RUNX2 and that RUNX2 exerted a negative regulatory effect on TALAM1 transcription.es
dc.description.urihttps://www-scopus-com.recursosbiblioteca.unab.cl/record/display.uri?eid=2-s2.0-85172088991&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&sid=acc61d57238ef2b32a8ffac8bb4c85c6&sot=aff&sdt=cl&cluster=scofreetoread%2c%22all%22%2ct&sl=34&s=AF-ID%2860002636%29+AND+SUBJAREA%28BIOC%29&relpos=12&citeCnt=0&searchTerm=
dc.identifier.citationCurrent Issues in Molecular Biology Open Access Volume 45, Issue 9, Pages 7075 - 7086 September 2023es
dc.identifier.doi10.3390/cimb45090447
dc.identifier.issn14673037
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53477
dc.language.isoenes
dc.publisherMDPIes
dc.rights.licenseCC BY 4.0 DEED
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleLong Noncoding RNA TALAM1 Is a Transcriptional Target of the RUNX2 Transcription Factor in Lung Adenocarcinomaes
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