Towards the bridging of molecular genetics data across Xenopus species

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dc.contributor.authorRiadi, G.
dc.contributor.authorOssandón, F.
dc.contributor.authorLarraín, J.
dc.contributor.authorMelo, F.
dc.date.accessioned2017-08-23T12:55:40Z
dc.date.available2017-08-23T12:55:40Z
dc.date.issued2016-03
dc.descriptionIndexación: Scopuses_CL
dc.description.abstractBackground: The clawed African frog Xenopus laevis has been one of the main vertebrate models for studies in developmental biology. However, for genetic studies, Xenopus tropicalis has been the experimental model of choice because it shorter life cycle and due to a more tractable genome that does not result from genome duplication as in the case of X. laevis. Today, although still organized in a large number of scaffolds, nearly 85 % of X. tropicalis and 89 % of X. laevis genomes have been sequenced. There is expectation for a comparative physical map that can be used as a Rosetta Stone between X. laevis genetic studies and X. tropicalis genomic research. Results: In this work, we have mapped using coarse-grained alignment the 18 chromosomes of X. laevis, release 9.1, on the 10 reference scaffolds representing the haploid genome of X. tropicalis, release 9.0. After validating the mapping with theoretical data, and estimating reference averages of genome sequence identity, 37 to 44 % between the two species, we have carried out a synteny analysis for 2,112 orthologous genes. We found that 99.6 % of genes are in the same organization. Conclusions: Taken together, our results make possible to establish the correspondence between 62 and 65.5 % of both genomes, percentage of identity, synteny and automatic annotation of transcripts of both species, providing a new and more comprehensive tool for comparative analysis of these two species, by allowing to bridge molecular genetics data among them.es_CL
dc.description.urihttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2440-9
dc.identifier.citationBMC Genomics. 2016. 17:161es_CL
dc.identifier.issn1471-2164
dc.identifier.otherhttps://doi.org/10.1186/s12864-016-2440-9
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/3998
dc.language.isoenes_CL
dc.publisherBIOMED CENTRALes_CL
dc.subjectXenopuses_CL
dc.subjectLaevises_CL
dc.subjectTropicalises_CL
dc.subjectAssemblyes_CL
dc.subjectCoarse-grainedes_CL
dc.subjectAlignmentes_CL
dc.subjectMapes_CL
dc.subjectSyntenyes_CL
dc.subjectGenomees_CL
dc.subjectSequenceses_CL
dc.titleTowards the bridging of molecular genetics data across Xenopus specieses_CL
dc.typeArtículoes_CL
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