A protocol for wide-scope non-target analysis of contaminants in small amounts of biota using bead beating tissuelyser extraction and LC-HRMS

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dc.contributor.authorGil-Solsona R.
dc.contributor.authorRodriguez-Mozaz S.
dc.contributor.authorDiaz-Cruz M.S.
dc.contributor.authorSunyer-Caldú A.
dc.contributor.authorLuarte T.
dc.contributor.authorHöfer J.
dc.contributor.authorGalbán-Malagón C.
dc.contributor.authorGago-Ferrero P.
dc.date.accessioned2025-01-28T15:28:37Z
dc.date.available2025-01-28T15:28:37Z
dc.date.issued2021
dc.descriptionIndexación: Scopus.
dc.description.abstractThis work describes a robust and powerful method for wide-scope target and non-target analysis of xenobiotics in biota samples based on bead beating tissuelyser extraction, solid phase extraction (SPE) clean-up and further detection by liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS). Unlike target methodologies, non-target methods usually aim at determining a wide range of still unknown substances with different physicochemical properties. Therefore, losses during the extraction process were minimised. Apart from that, the reduction of possible interferences showed to be necessary to expand the number of compounds that can be detected. This was achieved with an additional SPE clean-up step carried out with mixed-bed multi-layered cartridges. The method was validated with a set of 27 compounds covering a wide range of physicochemical properties, and further applied to the analysis of krill and fish samples. • The bead beating extraction was efficient for a wide range of organic pollutants in small quantities of biota samples. • Multi-layered solid phase extraction clean-up yield a wide xenobiotics coverage reducing matrix effects. • Method validation with 27 compounds led to a suitable method for non-target analysis of organic pollutants in biota. © 2020
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S2215016120304131?pes=vor&utm_source=scopus&getft_integrator=scopus
dc.identifier.doi10.1016/j.mex.2020.101193
dc.identifier.issn22150161
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/63323
dc.language.isoen
dc.publisherMethodsX, Volume 8 January 2021 Article number 101193
dc.rights.licenseAttribution 4.0 International CC BY 4.0 Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBead beating
dc.subjectBiota
dc.subjectHRMS
dc.subjectMixed-mode solid phase extraction
dc.subjectNon-target screening
dc.subjectNon-target wide-scope analysis of organic contaminants for small quantities of biota samples
dc.subjectSuspect screening
dc.titleA protocol for wide-scope non-target analysis of contaminants in small amounts of biota using bead beating tissuelyser extraction and LC-HRMS
dc.typeArtículo
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