Genome-Wide Characterization of Light-Regulated Gene Expression in Botrytis cinerea Reveals Underlying Complex Photobiology

Vista sencilla de ítem
dc.contributor.authorPérez-Lara, Gabriel
dc.contributor.authorOlivares-Yañez, Consuelo
dc.contributor.authorvan Bakel, Harm
dc.contributor.authorLarrondo, Luis F.
dc.contributor.authorCanessa, Paulo
dc.date.accessioned2024-10-17T16:29:30Z
dc.date.available2024-10-17T16:29:30Z
dc.date.issued2023-05
dc.descriptionIndexación: Scopus
dc.description.abstractBotrytis cinerea is a necrotrophic fungus characterized mainly by its wide host range of infected plants. The deletion of the white-collar-1 gene (bcwcl1), which encodes for a blue-light receptor/transcription factor, causes a decrease in virulence, particularly when assays are conducted in the presence of light or photocycles. However, despite ample characterization, the extent of the light-modulated transcriptional responses regulated by BcWCL1 remains unknown. In this study, pathogen and pathogen:host RNA-seq analyses, conducted during non-infective in vitro plate growth and when infecting Arabidopsis thaliana leaves, respectively, informed on the global gene expression patterns after a 60 min light pulse on the wild-type B05.10 or ∆bcwcl1 B. cinerea strains. The results revealed a complex fungal photobiology, where the mutant did not react to the light pulse during its interaction with the plant. Indeed, when infecting Arabidopsis, no photoreceptor-encoding genes were upregulated upon the light pulse in the ∆bcwcl1 mutant. Differentially expressed genes (DEGs) in B. cinerea under non-infecting conditions were predominantly related to decreased energy production in response to the light pulse. In contrast, DEGs during infection significantly differ in the B05.10 strain and the ∆bcwcl1 mutant. Upon illumination at 24 h post-infection in planta, a decrease in the B. cinerea virulence-associated transcripts was observed. Accordingly, after a light pulse, biological functions associated with plant defense appear enriched among light-repressed genes in fungus-infected plants. Taken together, our results show the main transcriptomic differences between wild-type B. cinerea B05.10 and ∆bcwcl1 after a 60 min light pulse when growing saprophytically on a Petri dish and necrotrophically over A. thaliana. © 2023 by the authors.
dc.description.urihttps://www.mdpi.com/1422-0067/24/10/8705
dc.identifier.citationInternational Journal of Molecular Sciences. Volume 24, Issue 10. May 2023. Article number 8705
dc.identifier.issn1661-6596
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/61350
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.rights.licenseCC BY 4.0 Attribution 4.0 International Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBotrytis Cinerea
dc.subjectLight Receptor
dc.subjectPhotoreceptor
dc.subjectPhytopathogen
dc.subjectTranscriptomics
dc.subjectWhite Collar
dc.titleGenome-Wide Characterization of Light-Regulated Gene Expression in Botrytis cinerea Reveals Underlying Complex Photobiology
dc.typeArtículo
Archivos
Bloque original
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
Perez-Lara_Genome-Wide_Characterization_of_Light-Regulated_2023.pdf
Tamaño:
4.43 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLES
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista