SNX5 promotes antigen presentation in B cells by dual regulation of actin and lysosomal dynamics

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dc.contributor.authorCabrera-Reyes, Fernanda
dc.contributor.authorContreras-Palacios, Teemly
dc.contributor.authorJara-Wilde, Jorge
dc.contributor.authorCaballero, Mia
dc.contributor.authorQuiroga, Clara
dc.contributor.authorFeijoo, Carmen G.
dc.contributor.authorDíaz-Muñoz, Jheimmy
dc.contributor.authorYuseff, María-Isabel
dc.date.accessioned2025-01-28T18:56:00Z
dc.date.available2025-01-28T18:56:00Z
dc.date.issued0025-01
dc.descriptionINDEXACION SCOPUS
dc.description.abstractB cells rapidly adapt their endocytic pathway to promote the uptake and processing of extracellular antigens recognized through the B-cell receptor (BCR). The mechanisms coupling changes in endomembrane trafficking to the capacity of B cells to screen for antigens within lymphoid tissues remain unaddressed. We investigated the role of SNX5, a member of the sorting nexin family, which interacts with endocytic membranes to regulate vesicular trafficking and macropinocytosis. Our results show that in steady state, B cells form SNX5-rich protrusions at the plasma membrane, which dissipate upon interaction with soluble antigens, whereas B cells activated with immobilized antigens accumulate SNX5 at the immune synapse where it regulates actin-dependent spreading responses. B cells silenced for SNX5 exhibit enlarged lysosomes, which are not recruited to the synaptic membrane, decreasing their capacity to extract immobilized antigens. Overall, our findings reveal that SNX5 is critical for actin-dependent plasma membrane remodeling in B cells in-volved in antigen screening and immune synapse formation, as well as endolysosomal trafficking required to promote antigen extraction and presentation. © 2024 Cabrera-Reyes et al.
dc.identifier.doi10.26508/lsa.202402917
dc.identifier.issn25751077
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/63332
dc.language.isoen
dc.publisherLife Science Alliance, LLC
dc.rights.licenseCC BY LICENSE
dc.subjectanimal; antigen presentation; B lymphocyte; cell membrane; endocytosis; endosome; genetics; human; immunological synapse; immunology; lysosome; metabolism; protein transport
dc.titleSNX5 promotes antigen presentation in B cells by dual regulation of actin and lysosomal dynamics
dc.typeArtículo
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