The NarE protein of neisseria gonorrhoeae catalyzes ADP-ribosylation of several ADP-ribose acceptors despite an N-terminal deletion

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dc.contributor.authorRodas, Paula I.
dc.contributor.authorÁlamos-Musre, A. Said
dc.contributor.authorÁlvarez, Francisca P.
dc.contributor.authorEscobar, Alejandro
dc.contributor.authorTapia, Cecilia V.
dc.contributor.authorOsorio, Eduardo
dc.contributor.authorOtero, Carolina
dc.contributor.authorCalderón, Iván L.
dc.contributor.authorFuentes, Juan A.
dc.contributor.authorGil, Fernando
dc.contributor.authorParedes-Sabja, Daniel
dc.contributor.authorChristodoulides, Myron
dc.date.accessioned2023-09-27T15:31:39Z
dc.date.available2023-09-27T15:31:39Z
dc.date.issued2016-09
dc.descriptionIndexación: Scopus.es
dc.description.abstractThe ADP-ribosylating enzymes are encoded in many pathogenic bacteria in order to affect essential functions of the host. In this study, we show that Neisseria gonorrhoeae possess a locus that corresponds to the ADP-ribosyltransferase NarE, a previously characterized enzyme in N. meningitidis. The 291 bp coding sequence of gonococcal narE shares 100% identity with part of the coding sequence of the meningococcal narE gene due to a frameshift previously described, thus leading to a 49-amino-acid deletion at the N-terminus of gonococcal NarE protein. However, we found a promoter region and a GTG start codon, which allowed expression of the protein as demonstrated by RT-PCR and western blot analyses. Using a gonococcal NarE–6xHis fusion protein, we demonstrated that the gonococcal enzyme underwent auto-ADP-ribosylation but to a lower extent than meningococcal NarE. We also observed that gonoccocal NarE exhibited ADP-ribosyltransferase activity using agmatine and cell-free host proteins as ADP-ribose acceptors, but its activity was inhibited by human β-defensins. Taken together, our results showed that NarE of Neisseria gonorrhoeae is a functional enzyme that possesses key features of bacterial ADP-ribosylating enzymes.es
dc.description.urihttps://academic-oup-com.recursosbiblioteca.unab.cl/femsle/article/363/17/fnw181/2197729
dc.identifier.citationFEMS Microbiology Letters. Volume 363, Issue 17. 1 September 2016. Article number fnw181es
dc.identifier.doi10.1093/femsle/fnw181
dc.identifier.issn0378-1097
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53342
dc.language.isoenes
dc.publisherOxford University Presses
dc.subjectNarEes
dc.subjectNeisseria Gonorrhoeaees
dc.subjectADP-Ribosyltransferasees
dc.titleThe NarE protein of neisseria gonorrhoeae catalyzes ADP-ribosylation of several ADP-ribose acceptors despite an N-terminal deletiones
dc.typeArtículoes
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