Whole-Genome Transcript Expression Profiling Reveals Novel Insights into Transposon Genes and Non-Coding RNAs during Atlantic Salmon Seawater Adaptation

Vista sencilla de ítem
dc.contributor.authorValentina Valenzuela-Muñoz
dc.contributor.authorCristian Gallardo-Escárate
dc.contributor.authorBárbara P. Benavente
dc.contributor.authorDiego Valenzuela-Miranda
dc.contributor.authorGustavo Núñez-Acuña
dc.contributor.authorHugo Escobar-Sepulveda
dc.contributor.authorJuan Antonio Váldes
dc.date.accessioned2022-10-11T21:01:36Z
dc.date.available2022-10-11T21:01:36Z
dc.date.issued2022-01
dc.description.abstractThe growing amount of genome information and transcriptomes data available allows for a better understanding of biological processes. However, analysis of complex transcriptomic experimental designs involving different conditions, tissues, or times is relevant. This study proposes a novel approach to analyze complex data sets combining transcriptomes and miRNAs at the chromosome-level genome. Atlantic salmon smolts were transferred to seawater under two strategies: (i) fish group exposed to gradual salinity changes (GSC) and (ii) fish group exposed to a salinity shock (SS). Gills, intestine, and head kidney samples were used for total RNA extraction, followed by mRNA and small RNA illumina sequencing. Different expression patterns among the tissues and treatments were observed through a whole-genome transcriptomic approach. Chromosome regions highly expressed between experimental conditions included a great abundance of transposable elements. In addition, differential expression analysis showed a greater number of transcripts modulated in response to SS in gills and head kidney. miRNA expression analysis suggested a small number of miRNAs involved in the smoltification process. However, target analysis of these miRNAs showed a regulatory role in growth, stress response, and immunity. This study is the first to evidence the interplaying among mRNAs and miRNAs and the structural relationship at the genome level during Atlantic salmon smoltification. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.description.sponsorshipIndexación: Scopuses
dc.identifier.citationMDPIes
dc.identifier.issn20797737
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/24231
dc.language.isoenes
dc.publisherMDPIes
dc.subjectAtlantic salmon; Genome; MiRNAs; MRNAs; Smoltificationes
dc.titleWhole-Genome Transcript Expression Profiling Reveals Novel Insights into Transposon Genes and Non-Coding RNAs during Atlantic Salmon Seawater Adaptationes
dc.typeArtículoes
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Valenzuela-Muñoz-WholeGenome-Transcript-Expression-Profiling-Reveals-Novel-Insights-into-Transposon-Genes-and-NonCoding-RNAs-during-Atlantic-Salmon-Seawater-AdaptationBiology.pdf
Tamaño:
7.7 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLÉS
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista