Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats

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dc.contributor.authorPacheco, A.
dc.contributor.authorAguayo, F.I.
dc.contributor.authorAliaga, E.
dc.contributor.authorMuñoz, M.
dc.contributor.authorGarcía-Rojo, G.
dc.contributor.authorOlave, F.A.
dc.contributor.authorParra-Fiedler, N.A.
dc.contributor.authorGarcía-Pérez, A.
dc.contributor.authorTejos-Bravo, M.
dc.contributor.authorRojas, P.S.
dc.contributor.authorParra, C.S.
dc.contributor.authorFiedler, J.L.
dc.date.accessioned2018-02-01T15:37:49Z
dc.date.available2018-02-01T15:37:49Z
dc.date.issued2017-08
dc.descriptionIndexación: Web of Science; Scopus.es_CL
dc.description.abstractPrevious studies in rats have demonstrated that chronic restraint stress triggers anhedonia, depressive-like behaviors, anxiety and a reduction in dendritic spine density in hippocampal neurons. In this study, we compared the effect of repeated stress on the expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptor subunits in dorsal and ventral hippocampus (VH). Adult male Sprague-Dawley rats were randomly divided into control and stressed groups, and were daily restrained in their motion (2.5 h/day) during 14 days. We found that chronic stress promotes an increase in c-Fos mRNA levels in both hippocampal areas, although it was observed a reduction in the immunoreactivity at pyramidal cell layer. Furthermore, Arc mRNAs levels were increased in both dorsal and VH, accompanied by an increase in Arc immunoreactivity in dendritic hippocampal layers. Furthermore, stress triggered a reduction in PSD-95 and NR1 protein levels in whole extract of dorsal and VH. Moreover, a reduction in NR2A/NR2B ratio was observed only in dorsal pole. In synaptosomal fractions, we detected a rise in NR1 in dorsal hippocampus (DH). By indirect immunofluorescence we found that NR1 subunits rise, especially in neuropil areas of dorsal, but not VH. In relation to AMPA receptor (AMPAR) subunits, chronic stress did not trigger any change, either in dorsal or ventral hippocampal areas. These data suggest that DH is more sensitive than VH to chronic stress exposure, mainly altering the expression of NMDA receptor (NMDAR) subunits, and probably favors changes in the configuration of this receptor that may influence the function of this area.es_CL
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fnmol.2017.00244/full
dc.identifier.citationFrontiers in Molecular Neuroscience. Volume 10, 10 August 2017, Article number 244es_CL
dc.identifier.issn1662-5099
dc.identifier.otherDOI: 10.3389/fnmol.2017.00244
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/5235
dc.language.isoenes_CL
dc.publisherFrontiers Mediaes_CL
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAMPA receptor subunitses_CL
dc.subjectArces_CL
dc.subjectC-Foses_CL
dc.subjectDorsal hippocampuses_CL
dc.subjectImmediately early geneses_CL
dc.subjectNMDA receptor subunitses_CL
dc.subjectStresses_CL
dc.subjectVentral hippocampuses_CL
dc.titleChronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Ratses_CL
dc.typeArtículoes_CL
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