Discovery and Characterization of the ddx41 Gene in Atlantic Salmon: Evolutionary Implications, Structural Functions, and Innate Immune Responses to Piscirickettsia salmonis and Renibacterium salmoninarum Infections

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dc.contributor.authorYañez, Alejandro J.
dc.contributor.authorBarrientos, Claudia A.
dc.contributor.authorIsla, Adolfo
dc.contributor.authorAguilar, Marcelo
dc.contributor.authorFlores-Martin, Sandra N.
dc.contributor.authorYuivar, Yassef
dc.contributor.authorOjeda, Adriana
dc.contributor.authorIbieta, Pablo
dc.contributor.authorHernández, Mauricio
dc.contributor.authorFigueroa, Jaime
dc.contributor.authorAvendaño-Herrera, Rubén
dc.contributor.authorMancilla, Marcos
dc.date.accessioned2024-08-10T14:27:40Z
dc.date.available2024-08-10T14:27:40Z
dc.date.issued2024
dc.descriptionIndexación: Scopus.
dc.description.abstractThe innate immune response in Salmo salar, mediated by pattern recognition receptors (PRRs), is crucial for defending against pathogens. This study examined DDX41 protein functions as a cytosolic/nuclear sensor for cyclic dinucleotides, RNA, and DNA from invasive intracellular bacteria. The investigation determined the existence, conservation, and functional expression of the ddx41 gene in S. salar. In silico predictions and experimental validations identified a single ddx41 gene on chromosome 5 in S. salar, showing 83.92% homology with its human counterpart. Transcriptomic analysis in salmon head kidney confirmed gene transcriptional integrity. Proteomic identification through mass spectrometry characterized three unique peptides with 99.99% statistical confidence. Phylogenetic analysis demonstrated significant evolutionary conservation across species. Functional gene expression analysis in SHK-1 cells infected by Piscirickettsia salmonis and Renibacterium salmoninarum indicated significant upregulation of DDX41, correlated with increased proinflammatory cytokine levels and activation of irf3 and interferon signaling pathways. In vivo studies corroborated DDX41 activation in immune responses, particularly when S. salar was challenged with P. salmonis, underscoring its potential in enhancing disease resistance. This is the first study to identify the DDX41 pathway as a key component in S. salar innate immune response to invading pathogens, establishing a basis for future research in salmonid disease resistance.
dc.identifier.citationInternational Journal of Molecular Sciences Open Access Volume 25, Issue 12June 2024 Article number 6346
dc.identifier.issn16616596
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/59160
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.subjectAtlantic salmon–pathogen communication
dc.subjectbacterial infection
dc.subjectddx41 gene
dc.subjectfunctional gene expression
dc.subjectinnate immune response
dc.subjectstructural–functional analysis
dc.titleDiscovery and Characterization of the ddx41 Gene in Atlantic Salmon: Evolutionary Implications, Structural Functions, and Innate Immune Responses to Piscirickettsia salmonis and Renibacterium salmoninarum Infections
dc.typeArtículo
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Discovery and Characterization of the ddx41 Gene in Atlantic Salmon: Evolutionary Implications, Structural Functions, and Innate Immune Responses to Piscirickettsia salmonis and Renibacterium salmoninarum Infections
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