The Root Hair Specific SYP123 Regulates the Localization of Cell Wall Components and Contributes to Rizhobacterial Priming of Induced Systemic Resistance

Vista sencilla de ítem
dc.contributor.authorRodriguez-Furlán, Cecilia
dc.contributor.authorSalinas-Grenet, Hernán
dc.contributor.authorSandoval, Omar
dc.contributor.authorRecabarren, Camilo
dc.contributor.authorArraño-Salinas, Paulina
dc.contributor.authorSoto-Alvear, Sylvana
dc.contributor.authorOrellana, Ariel
dc.contributor.authorBlanco-Herrera, Francisca
dc.date.accessioned2017-01-30T18:38:26Z
dc.date.available2017-01-30T18:38:26Z
dc.date.issued2016-07
dc.descriptionIndexación: Web of Science.es
dc.description.abstractRoot hairs are important for nutrient and water uptake and are also critically involved the interaction with soil inhabiting microbiota. Root hairs are tubular-shaped outgrowths that emerge from trichoblasts. This polarized elongation is maintained and regulated by a robust mechanism involving the endomembrane secretory and endocytic system. Members of the syntaxin family of SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) in plants (SYP), have been implicated in regulation of the fusion of vesicles with the target membranes in both exocytic and endocytic pathways. One member of this family. SYP123, is expressed specifically in the root hairs and accumulated in the growing tip region. This study shows evidence of the SYP123 role in polarized trafficking using knockout insertional mutant plants. We were able to observe defects in the deposition of cell wall proline rich protein PRP3 and cell wall polysaccharides. In a complementary strategy, similar results were obtained using a plant expressing a dominant negative soluble version of SYP123 (SP2 fragment) lacking the transmembrane domain. The evidence presented indicates that SYP123 is also regulating PRP3 protein distribution by recycling by endocytosis. We also present evidence that indicates that SYP123 is necessary for the response of roots to plant growth promoting rhizobacterium (PGPR) in order to trigger trigger induced systemic response (ISR). Plants with a defective SYP123 function were unable to mount a systemic acquired resistance in response to bacterial pathogen infection and ISR upon interaction with rhizobacteria. These results indicated that SYP123 was involved in the polarized localization of protein and polysaccharides in growing root hairs and that this activity also contributed to the establishment of effective plant defense responses. Root hairs represent very plastic structures were many biotic and abiotic factors can affect the number, anatomy and physiology of root hairs. Here, we presented evidence that indicates that interactions with soil PGPR could be closely regulated by signaling involving secretory and/or endocytic trafficking at the root hair tip as a quick way to response to changing environmental conditions.es
dc.description.urihttp://journal.frontiersin.org/article/10.3389/fpls.2016.01081/full
dc.identifier.citationFront. Plant Sci., 26 July 2016es
dc.identifier.issn1664-462X
dc.identifier.otherhttps://doi.org/10.3389/fpls.2016.01081
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/2793
dc.language.isoenes
dc.publisherFRONTIERS MEDIA SAes
dc.subjectSyntaxines
dc.subjectRhizobacteriaes
dc.subjectInduced systemic resistancees
dc.subjectSystemic acquired resistancees
dc.subjectCell walles
dc.subjectTraffickinges
dc.subjectPRP3es
dc.subjectPlant growth promoting rhizobacteriumes
dc.titleThe Root Hair Specific SYP123 Regulates the Localization of Cell Wall Components and Contributes to Rizhobacterial Priming of Induced Systemic Resistancees
dc.typeArtículoes
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Rodriguez_Furlan_The_Root_Hair_Specific.pdf
Tamaño:
4.97 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO INGLES
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista