Cyclosporin A-treated Dendritic Cells may affect the outcome of organ transplantation by decreasing CD4+CD25+ regulatory T cell proliferation

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dc.contributor.authorPino, Karina
dc.contributor.authorMichea, Paula
dc.contributor.authorSauma, Daniela
dc.contributor.authorAlba, Andrea
dc.contributor.authorMorales, Jorge
dc.contributor.authorBono Merino, María Rosa
dc.contributor.authorFierro, Alberto
dc.contributor.authorRosemblatt, Mario
dc.date.accessioned2014-05-22T16:31:33Z
dc.date.accessioned2016-05-23T21:11:04Z
dc.date.available2014-05-22T16:31:33Z
dc.date.available2016-05-23T21:11:04Z
dc.date.issued2010
dc.descriptionhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300010&lng=es&nrm=isoes
dc.description.abstractOne of the mechanisms for generation of tolerance involves immature dendritic cells (DCs) and a subpopulation of regulatory CD4+ CD25+ T lymphocytes (TREG). The purpose of this work was to analyze how Cyclosporine A (CsA), a widely used immunosuppressive drug, may affect TREG proliferation. Purified and activated murine DCs obtained from bone marrow precursors differentiated with rGMCSF were co-cultured with purified CFSE-labeled TREG from OTII mice, and their phenotype and proliferation analyzed by flow cytometry. Our data indicate that DCs differentiated in the presence of CsA show an altered phenotype, with a lower expression of MHC-II and a lower activating capacity. Additionally, these CsA-treated DCs show decreased production of IL-2 and IL-12 and increased IL-10 secretion when stimulated with LPS, indicating an effect on the polarization of the immune response. Interestingly, CsA-treated DCs show an anti-tolerogenic effect since they reduce the proliferation of TREG cells from 72 to 47%. Further inhibition to a 24% of TREG proliferation was obtained as a direct effect of CsA on TREG. In conclusion, the anti-tolerogenic effect of CsA should be considered in the planning of immunosuppression in the context of clinical transplantation.es
dc.identifier.citationBiol. Res. v.43 n.3 Santiago 2010es
dc.identifier.issn0716-9760
dc.identifier.otherhttp://dx.doi.org/10.4067/S0716-97602010000300010
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/2462
dc.language.isoenes
dc.publisherSociedad de Biología de Chilees
dc.subjectCD4+CD25+ regulatory T celles
dc.subjectCyclosporine Aes
dc.subjectDendritic cellses
dc.subjectTransplant tolerancees
dc.titleCyclosporin A-treated Dendritic Cells may affect the outcome of organ transplantation by decreasing CD4+CD25+ regulatory T cell proliferationes
dc.typeArtículoes
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