Comparative EST transcript profiling of peach fruits under different post-harvest conditions reveals candidate genes associated with peach fruit quality

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dc.contributor.authorVizoso, Paula
dc.contributor.authorMeisel, Lee A.
dc.contributor.authorTittarelli, Andrés
dc.contributor.authorLatorre, Mariano
dc.contributor.authorSaba, Juan
dc.contributor.authorCaroca, Rodrigo
dc.contributor.authorMaldonado, Jonathan
dc.contributor.authorCambiazo, Veronica
dc.contributor.authorCampos-Vargas, Reinaldo
dc.contributor.authorGonzalez, Mauricio
dc.contributor.authorOrellana, Ariel
dc.contributor.authorSilva, Herman
dc.date.accessioned2024-06-25T00:39:13Z
dc.date.available2024-06-25T00:39:13Z
dc.date.issued2009-09-10
dc.descriptionINDEXACIÓN: SCOPUS.
dc.description.abstractBackground: Cold storage is used to inhibit peach fruit ripening during shipment to distant markets. However, this cold storage can negatively affect the quality of the fruit when it is ripened, resulting in disorders such as wooliness, browning or leathering. In order to understand the individual and combined biological effects that factors such as cold storage and ripening have on the fruit and fruit quality, we have taken a comparative EST transcript profiling approach to identify genes that are differentially expressed in response to these factors. Results: We sequenced 50,625 Expressed Sequence Tags (ESTs) from peach mesocarp (Prunus persica O'Henry variety) stored at four different postharvest conditions. A total of 10,830 Unigenes (4,169 contigs and 6,661 singletons) were formed by assembling these ESTs. Additionally, a collection of 614 full-length and 1,109 putative full-length cDNA clones within flanking loxP recombination sites was created. Conclusion: Statistically analyzing the EST population, we have identified genes that are differentially expressed during ripening, in response to cold storage or the combined effects of cold storage and ripening. Pair-wise comparisons revealed 197 contigs with at least one significant difference in transcript abundance between at least two conditions. Gene expression profile analyses revealed that the contigs may be classified into 13 different clusters of gene expression patterns. These clusters include groups of contigs that increase or decrease transcript abundance during ripening, in response to cold or ripening plus cold. These analyses have enabled us to statistically identify novel genes and gene clusters that are differentially expressed in response to post-harvest factors such as long-term cold storage, ripening or a combination of these two factors. These differentially expressed genes reveal the complex biological processes that are associated with these factors, as well as a large number of putative gene families that may participate differentially in these processes. In particular, these analyzes suggest that woolly fruits lack the increased boost of metabolic processes necessary for ripening. Additionally, these results suggest that the mitochondria and plastids play a major role in these processes. The EST sequences and full-length cDNA clones developed in this work, combined with the large population of differentially expressed genes may serve as useful tools and markers that will enable the scientific community to better define the molecular processes that affect fruit quality in response to post-harvest conditions and the organelles that participate in these processes. © 2009 Vizoso et al; licensee BioMed Central Ltd.
dc.identifier.citationBMC Genomics, Volume 10, Pages 423, 10 September 2009, Article number 1471
dc.identifier.doi10.1186/1471-2164-10-423
dc.identifier.issn1471-2164
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/57953
dc.language.isoen
dc.publisherBMC
dc.rights.licenseCC BY 2.0 ATTRIBUTION 2.0 GENERIC Deed
dc.rights.urihttps://creativecommons.org/licenses/by/2.0/
dc.subjectCluster Analysis
dc.subjectCold Temperature
dc.subjectComparative Genomic Hybridization
dc.subjectDNA, Plant
dc.subjectExpressed Sequence Tags
dc.subjectFruit
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Plant
dc.subjectGene Library
dc.subjectGenes, Plant
dc.subjectPrunus
dc.subjectSequence Analysis, DNA
dc.subjectThioxanthenes
dc.titleComparative EST transcript profiling of peach fruits under different post-harvest conditions reveals candidate genes associated with peach fruit quality
dc.typeArtículo
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