High‐Temperature Stress Effect on the Red Cusk‐Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damage

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dc.contributor.authorDettleff, Phillip
dc.contributor.authorZuloaga, Rodrigo
dc.contributor.authorFuentes, Marcia
dc.contributor.authorGonzalez, Pamela
dc.contributor.authorAedo, Jorge
dc.contributor.authorEstrada, Juan Manuel
dc.contributor.authorMolina, Alfredo
dc.contributor.authorValdés, Juan Antonio
dc.date.accessioned2023-10-13T22:51:23Z
dc.date.available2023-10-13T22:51:23Z
dc.date.issued2022-06
dc.descriptionIndexación: Scopuses
dc.description.abstractEnvironmental stressors, such as temperature, are relevant factors that could generate a negative effect on several tissues in fish. A key fish species for Chilean aquaculture diversification is the red cusk‐eel (Genypterus chilensis), a native fish for which knowledge on environmental stressors effects is limited. This study evaluated the effects of high‐temperature stress on the liver of red cusk‐eel in control (14 °C) and high‐temperature (19 °C) groups using multiple approaches: determination of plasmatic hepatic enzymes (ALT, AST, and AP), oxidative damage evaluation (AP sites, lipid peroxidation, and carbonylated proteins), and RNA‐seq analysis. High-temperature stress generated a significant increase in hepatic enzyme activity in plasma. In the liver, a transcriptional regulation was observed, with 1239 down‐regulated and 1339 up‐regulated transcripts. Additionally, high‐temperature stress generated oxidative stress in the liver, with oxidative damage and transcriptional modulation of the antioxidant response. Furthermore, an unfolded protein response was observed, with several pathways enriched, as well as a heat shock response, with several heat shock proteins up regulated, suggesting candidate biomarkers (i.e., serpinh1) for thermal stress evaluation in this species. The present study shows that high-temperature stress generated a major effect on the liver of red cusk‐eel, knowledge to consider for the aquaculture and fisheries of this species. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.description.urihttps://www.mdpi.com/2079-7737/11/7/990
dc.identifier.citationBiology Open Access Volume 11, Issue 7June 2022 Article number 990es
dc.identifier.doi10.3390/biology11070990
dc.identifier.issn2079-7737
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53464
dc.language.isoenes
dc.publisherMDPIes
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectHepatic enzymeses
dc.subjectLiver transcriptomees
dc.subjectOxidative damagees
dc.subjectProtein foldinges
dc.subjectRed cusk‐eeles
dc.subjectRNA‐seqes
dc.subjectThermal stresses
dc.titleHigh‐Temperature Stress Effect on the Red Cusk‐Eel (Geypterus chilensis) Liver: Transcriptional Modulation and Oxidative Stress Damagees
dc.typeArtículoes
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