Genetic analysis of antibiotic-resistance determinants in multidrug-resistant Shigella strains isolated from Chilean children
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Archivos
Fecha
2005-02
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Cambridge University Press
Nombre de Curso
Licencia CC
CC BY-NC-SA 4.0 DEED
Attribution-NonCommercial-ShareAlike 4.0 International
Licencia CC
https://creativecommons.org/licenses/by-nc-sa/4.0/deed.en
Resumen
A total of 162 clinical isolates of Shigella collected from children in a semi-rural community of Chile were examined for the presence of genetic determinants of resistance to ampicillin, chloramphenicol, tetracycline, and trimethoprim. Ampicillin resistance was most frequently associated with the presence of blaOXA in S. flexneri and with blaTEM in S. sonnei. The blaOXA gene but not blaTEM was located in class 1 integrons. The dhfrla gene encoding for resistance to trimethoprim was associated to class 2 integrons and detected exclusively in S. flexneri, whereas dhfrIIIc was found in all S. sonnei strains and in 10% of the S. flexneri isolates. Cat, coding for choramphenicol resistance, and blaOXA genes were located in the chromosome in all cases, whereas tetA gene, coding for tetracycline resistance, and blaTEM, dhfrIa and dhfrIIIc genes were found either in the chromosome or in conjugative plasmids. Our results show a heterogenous distribution of antibiotic-resistance determinants between S. flexneri and S. sonnei. © 2004 Cambridge University Press.
Notas
INDEXACIÓN: SCOPUS.
Palabras clave
Blotting, Southern, Child, Chile, Drug Resistance, Multiple, Bacterial, Dysentery, Bacillary, Female, Humans, Male, Plasmids, Polymerase Chain Reaction, Rural Population, Shigella flexneri, Shigella sonnei
Citación
Epidemiology and Infection, Volume 133, Issue 1, Pages 81 - 86, February 2005
DOI
10.1017/S0950268804003048