Design of a New Vaccine Prototype against Porcine Circovirus Type 2 (PCV2), M. hyopneumoniae and M. hyorhinis Based on Multiple Antigens Microencapsulation with Sulfated Chitosan

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dc.contributor.authorArrieta-Mendoza, Darwuin
dc.contributor.authorGarces, Bruno
dc.contributor.authorHidalgo, Alejandro A.
dc.contributor.authorNeira, Victor
dc.contributor.authorRamirez, Galia
dc.contributor.authorNeira-Carrillo, Andrónico
dc.contributor.authorBucarey, Sergio A.
dc.date.accessioned2024-07-19T14:07:11Z
dc.date.available2024-07-19T14:07:11Z
dc.date.issued2024-05
dc.descriptionIndexación: Scopus.
dc.description.abstractThis work evaluated in vivo an experimental-multivalent-vaccine (EMV) based on three Porcine Respiratory Complex (PRC)-associated antigens: Porcine Circovirus Type 2 (PCV2), M. hyopneumoniae (Mhyop) and M. hyorhinis (Mhyor), microencapsulated with sulfated chitosan (M- ChS + PRC-antigens), postulating chitosan sulphate (ChS) as a mimetic of the heparan sulfate receptor used by these pathogens for cell invasion. The EMV was evaluated physicochemically by SEM (Scanning-Electron-Microscopy), EDS (Energy-Dispersive-Spectroscopy), Pdi (Polydispersity-Index) and zeta potential. Twenty weaned pigs, distributed in four groups, were evaluated for 12 weeks. The groups 1 through 4 were as follows: 1-EMV intramuscular-route (IM), 2-EMV oral-nasal-route (O/N), 3-Placebo O/N (M-ChS without antigens), 4-Commercial-vaccine PCV2-Mhyop. qPCR was used to evaluate viral/bacterial load from serum, nasal and bronchial swab and from inguinal lymphoid samples. Specific humoral immunity was evaluated by ELISA. M-ChS + PRC-antigens measured between 1.3–10 μm and presented low Pdi and negative zeta potential, probably due to S (4.26%). Importantly, the 1-EMV protected 90% of challenged animals against PCV2 and Mhyop and 100% against Mhyor. A significant increase in antibody was observed for Mhyor (1-EMV and 2-EMV) and Mhyop (2-EMV), compared with 4-Commercial-vaccine. No difference in antibody levels between 1-EMV and 4-Commercial-vaccine for PCV2-Mhyop was observed. Conclusion: The results demonstrated the effectiveness of the first EMV with M-ChS + PRC-antigens in pigs, which were challenged with Mhyor, PCV2 and Mhyop, evidencing high protection for Mhyor, which has no commercial vaccine available.
dc.description.accesoabiertoSI
dc.description.agradThis study was financially supported by the Chilean Agency for Research and Development (ANID) Fondef Idea I + D project N◦ ID19I10135 granted to S.A. Bucarey and Fondo de Investigación Veterinaria de la Facultad de Cs. Veterinarias y Pecuarias de la Universidad de Chile (FIV2022) project N◦ 12101701 granted to S.A. Bucarey. PhD student, D. Arrieta is grateful for the funding provided by program Doctorate in Forestry, Agricultural and Veterinary Sciences, University of Chile, for the fee exemption scholarship from this accredited program.
dc.description.urihttps://www.mdpi.com/2076-393X/12/5/550
dc.identifier.citationVaccines. Open Access. Volume 12, Issue 5. May 2024. Article number 550
dc.identifier.doi10.3390/vaccines12050550
dc.identifier.folioChilean Agency for Research and Development (ANID) Fondef Idea I + D project N° ID19I10135
dc.identifier.generoH
dc.identifier.issn2076-393X
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/58601
dc.language.isoen
dc.other.orcidhttps://orcid.org/0000-0002-7788-3185
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.rights.licenseCC BY 4.0 Attribution 4.0 International Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectchitosan sulphated
dc.subjectheparan sulfate receptors
dc.subjectmicroparticles
dc.subjectmimetic
dc.subjectMycoplama hyopneumoniae
dc.subjectMycoplama hyorhinis
dc.subjectPCV2
dc.subjectPRC
dc.subjectvaccine
dc.titleDesign of a New Vaccine Prototype against Porcine Circovirus Type 2 (PCV2), M. hyopneumoniae and M. hyorhinis Based on Multiple Antigens Microencapsulation with Sulfated Chitosan
dc.typeArtículo
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Design-of-a-New-Vaccine-Prototype-against-Porcine-Circovirus-Type-2-PCV2-M-hyopneumoniae-and-M-hyorhinis-Based-on-Multiple-Antigens-Microencapsulation-with-Sulfated-Chitosan.pdf
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