Immunization with antigen-pulsed dendritic cells significantly improves the immune response to weak self-antigens

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dc.contributor.authorVargas, Pablo
dc.contributor.authorCortés, Claudio
dc.contributor.authorVargas, Leonardo
dc.contributor.authorRosemblatt, Mario
dc.contributor.authorBono, María Rosa
dc.date.accessioned2025-05-16T17:11:58Z
dc.date.available2025-05-16T17:11:58Z
dc.date.issued2006-02
dc.descriptionIndexación: Scopus
dc.description.abstractDendritic cells (DCs) are the only professional antigen-presenting cells endowed with the ability to stimulate naïve T cells and initiate a primary immune response. For this reason, DC-based immunization has been shown to be highly effective in eliciting CTL responses to viruses and tumor-associated antigens. Here we report on the use of DC immunization to enhance the B cell-mediated humoral immune response to highly conserved proteins and the application of this approach to the generation of monoclonal antibodies (mAbs) against these proteins. To illustrate the technique we describe the production of mAbs to class II transactivator (CIITA), the major histocompatibility complex (MHC) CIITA, a difficult immunogen owing to its high degree of identity among species. We show that mice immunized with a combination of an intravenous injection of DCs pulsed with recombinant fragments of CIITA followed by intraperitoneal injection of the antigen in incomplete Freund's adjuvant induced a detectable antibody response against CIITA, while sera from mice immunized using the traditional method (i.e. intraperitoneal immunization with 50 μg of protein in complete Freund's adjuvant) gave an almost undetectable response. Furthermore, a total of four fusion experiments demonstrate that immunization with Ag-pulsed DCs is necessary for the efficient generation of hybridomas and a good yield of mAbs specific for the recombinant and the native endogenous CIITA protein. Conversely, four independent fusions carried out with splenocytes from mice immunized using the traditional method failed to produce anti-CIITA hybridomas. We propose that immunization with antigen-loaded DCs should be the method of preference when attempting to raise mAbs against weak self-immunogens. © 2005 Elsevier GmbH. All rights reserved.
dc.description.accesoabiertoSi
dc.description.agradProyectos ANID: FONDECYT (1030875, 1030074 and 20000-062) to MRB, MR and CC. Supported also by MIFAB and Fundación Andes
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S0171298505001683?via%3Dihub
dc.identifier.citationImmunobiology. Volume 211, Issue 1-2, Pages 29 - 36. 22 February 2006
dc.identifier.doi10.1016/j.imbio.2005.09.002
dc.identifier.folioFONDECYT 1030875
dc.identifier.folioFONDECYT 1030074
dc.identifier.folioFONDECYT 20000-062
dc.identifier.generoH
dc.identifier.issn0171-2985
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/64730
dc.language.isoen
dc.publisherElsevier GmbH
dc.subjectClass II transactivator (CIITA)
dc.subjectDendritic Cells
dc.subjectImmune Response
dc.subjectImmunization
dc.subjectMonoclonal Antibodies
dc.titleImmunization with antigen-pulsed dendritic cells significantly improves the immune response to weak self-antigens
dc.typeArtículo
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