Global gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosa

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dc.contributor.authorPastenes, L.
dc.contributor.authorValdivieso, C.
dc.contributor.authorDi Genova, A.
dc.contributor.authorTravisany, D.
dc.contributor.authorHart, A.
dc.contributor.authorMontecino, M.
dc.contributor.authorOrellana, A.
dc.contributor.authorGonzalez, M.
dc.contributor.authorGutiérrez, R.A.
dc.contributor.authorAllende, M.L.
dc.contributor.authorMaass, A.
dc.contributor.authorMéndez, M.A.
dc.date.accessioned2017-11-24T13:46:40Z
dc.date.available2017-11-24T13:46:40Z
dc.date.issued2017-05
dc.descriptionIndexación: Web of Science; Scopus.es_CL
dc.description.abstractThe anuran Rhinella spinulosa is distributed along the Andes Range at altitudes that undergo wide daily and seasonal variation in temperature. One of the populations inhabits geothermal streams, a stable environment that influences life history traits such as the timing of metamorphosis. To investigate whether this population has undergone local adaptation to this unique habitat, we carried out transcriptome analyses in animals from two localities in two developmental stages (prometamorphic and metamorphic) and exposed them to two temperatures (20 and 25 degrees C). RNA-Seq, de novo assembly and annotation defined a transcriptome revealing 194,469 high quality SNPs, with 1,507 genes under positive selection. Comparisons among the experimental conditions yielded 1,593 differentially expressed genes. A bioinformatics search for candidates revealed a total of 70 genes that are highly likely to be implicated in the adaptive response of the population living in a stable environment, compared to those living in an environment with variable temperatures. Most importantly, the population inhabiting the geothermal environment showed decreased transcriptional plasticity and reduced genetic variation compared to its counterpart from the non-stable environment. This analysis will help to advance the understanding of the molecular mechanisms that account for the local adaptation to geothermal streams in anurans.es_CL
dc.description.urihttps://www.nature.com/articles/s41598-017-01982-z
dc.identifier.citationScientific Reports. Volume 7, Issue 1, December 2017, Article number 1966es_CL
dc.identifier.issn2045-2322
dc.identifier.otherdoi:10.1038/s41598-017-01982-z
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/4720
dc.language.isoenes_CL
dc.publisherNature Publishing Groupes_CL
dc.subjectFROG RANA-TEMPORARIAes_CL
dc.subjectANURAN LARVAL GROWTHes_CL
dc.subjectLIFE-HISTORY TRAITSes_CL
dc.subjectCORAL-REEF FISHes_CL
dc.subjectCOMMON FROGes_CL
dc.subjectRNA-SEQes_CL
dc.subjectTEMPERATUREes_CL
dc.subjectBUFONIDAEes_CL
dc.subjectMETAMORPHOSISes_CL
dc.subjectBUFOes_CL
dc.titleGlobal gene expression analysis provides insight into local adaptation to geothermal streams in tadpoles of the Andean toad Rhinella spinulosaes_CL
dc.typeArtículoes_CL
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