Antimicrobial and antibiofilm capacity of chitosan nanoparticles against wild type strain of pseudomonas sp. Isolated from milk of cows diagnosed with bovine mastitis

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dc.contributor.authorAguayo, P.R.
dc.contributor.authorLarenas, T.B.
dc.contributor.authorGodoy, C.A.
dc.contributor.authorRivas, B.C.
dc.contributor.authorGonzalez-Casanova, J.
dc.contributor.authorRojas-Gomez, D.
dc.contributor.authorFuentes, N.C.
dc.date.accessioned2021-07-30T13:24:52Z
dc.date.available2021-07-30T13:24:52Z
dc.date.issued2020-09
dc.descriptionIndexación; Scopus.es
dc.description.abstractBovine mastitis (BM) is the most prevalent bacterial infection in the livestock sector, affecting the dairy industry greatly. The prevention and treatment of this disease is mainly made via antibiotics, but the increasing antimicrobial resistance of pathogens has affected the efficiency of conventional drugs. Pseudomonas sp. is one of the pathogens involved in this infection. The therapeutic rate of cure for this environmental mastitis-causing pathogen is practically zero, regardless of treatment. Biofilm formation has been one of the main virulence mechanisms of Pseudomonas hence presenting resistance to antibiotic therapy. We have manufactured chitosan nanoparticles (NQo) with tripolyphosphate (TPP) using ionotropic gelation. These NQo were confronted against a Pseudomonas sp. strain isolated from milk samples of cows diagnosed with BM, to evaluate their antimicrobial and antibiofilm capacity. The NQo showed great antibacterial effect in the minimum inhibitory concentrations (MIC), minimum bactericidal concentration (MBC) and disk diffusion assays. Using sub lethal concentrations, NQo were tested for inhibition of biofilm formation. The results show that the nanoparticles exhibited biofilm inhibition and were capable of eradicate pre-existing mature biofilm. These findings indicate that the NQo could act as a potential alternative to antibiotic treatment of BM. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.description.urihttps://www.mdpi.com/2079-6382/9/9/551
dc.identifier.citationAntibioticsOpen AccessVolume 9, Issue 9, Pages 1 - 15September 2020 Article number 551es
dc.identifier.doi10.3390/antibiotics9090551
dc.identifier.issn2079-6382
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/19582
dc.language.isoenes
dc.publisherAntibioticses
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAntibiofilmes
dc.subjectBovine mastitises
dc.subjectChitosanes
dc.subjectNanoparticleses
dc.subjectPseudomonases
dc.titleAntimicrobial and antibiofilm capacity of chitosan nanoparticles against wild type strain of pseudomonas sp. Isolated from milk of cows diagnosed with bovine mastitises
dc.typeArtículoes
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