Host–virus interactions during infection with a wild-type ILTV strain or a glycoprotein G deletion mutant ILTV vaccine strain in an ex vivo system

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dc.contributor.authorGopakumar, Gayathri
dc.contributor.authorCoppo, Mauricio J.C.
dc.contributor.authorDiaz-Méndez, Andrés
dc.contributor.authorHartley, Carol A.
dc.contributor.authorDevlin, Joanne M.
dc.date.accessioned2025-03-14T17:31:29Z
dc.date.available2025-03-14T17:31:29Z
dc.date.issued2025-02
dc.descriptionIndexación (Scopus)
dc.description.abstractPrevious studies have demonstrated the safety and efficacy of a live-attenuated glycoprotein G (gG) deletion mutant vaccine strain of ILTV (∆gG-ILTV). In the current study, transcriptional profiles of chicken tracheal organ cultures (TOCs), 24 h post inoculation with ∆gG-ILTV or the gG-expressing parent wild-type strain, CSW-1 ILTV were explored and compared with the mock-infected TOCs using RNA-seq analysis. Transcriptomes of the vaccine and wild-type ILTV were also compared with each other. Although no viral genes (except for gG) were differentially regulated between the two ILTV-infected TOCs, pair-wise comparison of the transcriptomes of the ∆gG-ILTV or the CSW-1 ILTV-infected TOCs (each compared with mock-infected TOCs) identified the similarities and differences in host gene transcription between them. Several immune checkpoint inhibitors with likely roles in ILTV-mediated immune augmentation, and gene ontologies indicating cytokine response, and cytokine signaling were upregulated in both TOCs. Additionally, several other biological processes, molecular functions, and cellular components were enriched uniquely in the ∆gG-ILTV-infected TOCs, including those that indicated modifications to tracheal extracellular matrix (ECM) structural components, which may have a role in immune modulation in vivo. This study has revealed that the modifications of transcription of host genes during the early stages of ILTV infection are not limited to changes in cytokine or chemokine gene transcription, but several other immune-related genes and ECM components. Moreover, their differential regulation in the ex vivo system appears to be influenced by gG expression, potentially affecting the outcome of ILTV infection in vivo.
dc.description.urihttps://journals.asm.org/doi/epdf/10.1128/spectrum.01183-24?src=getftr&utm_source=scopus&getft_integrator=scopus
dc.identifier.citationMicrobiology Spectrum Volume 13, Issue 2 February 2025
dc.identifier.doi10.1128/spectrum.01183-24
dc.identifier.issn2165-0497
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/63779
dc.language.isoen_US
dc.publisherAmerican Society for Microbiology
dc.subject2165-0497
dc.titleHost–virus interactions during infection with a wild-type ILTV strain or a glycoprotein G deletion mutant ILTV vaccine strain in an ex vivo system
dc.typeArtículo
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