The genome sequence of the soft-rot fungus Penicillium purpurogenum reveals a high gene dosage for lignocellulolytic enzymes
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Fecha
2018-01
Profesor/a Guía
Facultad/escuela
Idioma
en
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Editor
Taylor and Francis Ltd.
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CC BY-NC
CC BY-NC-ND
Licencia CC
Resumen
The high lignocellulolytic activity displayed by the soft-rot fungus Penicillium purpurogenum has made it a target for the study of novel lignocellulolytic enzymes. We have obtained a reference genome of 36.2 Mb of non-redundant sequence (11,057 protein-coding genes). The 49 largest scaffolds cover 90% of the assembly, and Core Eukaryotic Genes Mapping Approach (CEGMA) analysis reveals that our assembly captures almost all protein-coding genes. RNA-seq was performed and 93.1% of the reads aligned to the assembled genome. These data, plus the independent sequencing of a set of genes of lignocellulose-degrading enzymes, validate the quality of the genome sequence. P. purpurogenum shows a higher number of proteins with CAZy motifs, transcription factors and transporters as compared to other sequenced Penicillia. These results demonstrate the great potential for lignocellulolytic activity of this fungus and the possible use of its enzymes in related industrial applications. © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Notas
Indexación Scopus
Palabras clave
Exons, Prediction, Molecular Sequence Annotation, lignocellulose biodegradation, Penicillium purpurogenum, RNA-seq
Citación
Mycology Volume 9, Issue 1, Pages 59 - 692 January 2018
DOI
10.1080/21501203.2017.1419995