Catabolic Signaling Pathways, Atrogenes, and Ubiquitinated Proteins Are Regulated by the Nutritional Status in the Muscle of the Fine Flounder

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dc.contributor.authorFuentes, E.
dc.contributor.authorRuiz, P.
dc.contributor.authorValdes, J.
dc.contributor.authorMolina, A.
dc.date.accessioned2023-06-13T16:18:53Z
dc.date.available2023-06-13T16:18:53Z
dc.date.issued2012-09
dc.descriptionIndexación: Scopus.es
dc.description.abstractA description of the intracellular mechanisms that modulate skeletal muscle atrophy in early vertebrates is still lacking. In this context, we used the fine flounder, a unique and intriguing fish model, which exhibits remarkably slow growth due to low production of muscle-derived IGF-I, a key growth factor that has been widely acknowledged to prevent and revert muscle atrophy. Key components of the atrophy system were examined in this species using a detailed time-course of sampling points, including two contrasting nutritional periods. Under basal conditions high amounts of the atrogenes MuRF-1 and Atrogin-1 were observed. During fasting, the activation of the P38/MAPK and Akt/FoxO signaling pathways decreased; whereas, the activation of the IκBα/NFκB pathway increased. These changes in signal transduction activation were concomitant with a strong increase in MuRF-1, Atrogin-1, and protein ubiquitination. During short-term refeeding, the P38/MAPK and Akt/FoxO signaling pathways were strongly activated, whereas the activation of the IκBα/NFκB pathway decreased significantly. The expression of both atrogenes, as well as the ubiquitination of proteins, dropped significantly during the first hour of refeeding, indicating a strong anti-atrophic condition during the onset of refeeding. During long-term refeeding, Akt remained activated at higher than basal levels until the end of refeeding, and Atrogin-1 expression remained significantly lower during this period. This study shows that the components of the atrophy system in skeletal muscle appeared early in the evolution of vertebrates and some mechanisms have been conserved, whereas others have not. These results represent an important achievement for the area of fish muscle physiology, showing an integrative view of the atrophy system in a non-mammalian species and contributing to novel insights on the molecular basis of muscle growth regulation in earlier vertebrates.es
dc.description.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0044256
dc.identifier.citationPLoS ONE, Volume 7, Issue 914, September 2012, Article number e44256es
dc.identifier.doi10.1371/journal.pone.0044256
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/50613
dc.language.isoenes
dc.publisherPublic Library of Science (PLoS)es
dc.rights.licenseAttribution 2.0 Generic (CC BY 2.0)
dc.rights.urihttps://plos.org/open-science/open-access/
dc.subjectGrowth Hormonees
dc.subjectFishes
dc.subjectSomatomedinses
dc.subjectenzyme activationes
dc.subjectfeeding behaviores
dc.subjectflounderes
dc.subjectgene expression regulationes
dc.titleCatabolic Signaling Pathways, Atrogenes, and Ubiquitinated Proteins Are Regulated by the Nutritional Status in the Muscle of the Fine Flounderes
dc.typeArtículoes
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