Effects of crowding on the three main proteolytic mechanisms of skeletal muscle in rainbow trout (Oncorhynchus mykiss)

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dc.contributor.authorValenzuela, Cristián A.
dc.contributor.authorPonce, Claudia
dc.contributor.authorZuloaga, Rodrigo
dc.contributor.authorGonzález, Pamela
dc.contributor.authorAvendaño-Herrera, Ruben
dc.contributor.authorValdés, Juan A.
dc.contributor.authorMolina, Alfredo
dc.date.accessioned2022-06-14T20:40:29Z
dc.date.available2022-06-14T20:40:29Z
dc.date.issued2020-08
dc.descriptionIndexación: Scopus.es
dc.description.abstractBackground: Skeletal muscle is one of the tissues most affected by stress conditions. The protein degradation in this tissue is vital for the supply of energy mediated by different proteolytic pathways such as the ubiquitin-proteasome (UPS), autophagy-lysosome (ALS) and the calpain/calpastatin system (CCS). Nevertheless, the regulation of this proteolytic axis under stress conditions is not yet completely clear. Chile is the main producer of rainbow trout (Oncorhynchus mykiss) in the world. This intensive fish farming has resulted in growing problems as crowding and stress are one of the major problems in the freshwater stage. In this context, we evaluated the crowding effect in juvenile rainbow trout kept in high stocking density (30 kg/m3) for 15, 45 and 60 days, using a control group of fish (10 kg/m3). Results: Plasmatic cortisol and glucose were evaluated by enzyme immunoassay. The mRNA levels of stress-related genes (gr1, gr2, mr, hsp70, klf15 and redd1), markers of the UPS (atrogin1 and murf1) and CCS (capn1, capn1, cast-l and cast-s) were evaluated using qPCR. ALS (LC3-I/II and P62/SQSTM1) and growth markers (4E-BP1 and ERK) were measured by Western blot analysis. The cortisol levels increased concomitantly with weight loss at 45 days of crowding. The UPS alone was upregulated at 15 days of high stocking density, while ALS activation was observed at 60 days. However, the CCS was inactivated during the entire trial. Conclusion: All these data suggest that stress conditions, such as crowding, promote muscle degradation in a time-dependent manner through the upregulation of the UPS at early stages of chronic stress and activation of the ALS in long-term stress, while the CCS is strongly inhibited by stress conditions in the rainbow trout muscle farmed during freshwater stage. Our descriptive study will allow perform functional analysis to determine, in a more detailed way, the effect of stress on skeletal muscle physiology as well as in the animal welfare in rainbow trout. Moreover, it is the first step to elucidate the optimal crop density in the freshwater stage and improve the standards of Chilean aquaculture. © 2020 The Author(s).es
dc.description.urihttps://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02518-w
dc.identifier.citationBMC Veterinary Research Open Access Volume 16, Issue 117 August 2020 Article number 294es
dc.identifier.doi10.1186/s12917-020-02518-w
dc.identifier.issn17466148
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/22831
dc.language.isoenes
dc.publisherBioMed Central Ltdes
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAutophagyes
dc.subjectCalpain/calpastatines
dc.subjectFishes
dc.subjectStress responsees
dc.subjectUbiquitin-proteasomees
dc.titleEffects of crowding on the three main proteolytic mechanisms of skeletal muscle in rainbow trout (Oncorhynchus mykiss)es
dc.typeArtículoes
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