Motility modulation by the small non-coding RNA SroC in Salmonella Typhimurium

Vista sencilla de ítem
dc.contributor.authorFuentes, Danitza N.
dc.contributor.authorCalderón, Paulina F.
dc.contributor.authorAcuña, Lillian G.
dc.contributor.authorRodas, Paula I.
dc.contributor.authorParedes-Sabja, Daniel
dc.contributor.authorFuentes, Juan A.
dc.contributor.authorGil, Fernando
dc.contributor.authorCalderón, Iván L.
dc.date.accessioned2023-03-21T14:42:55Z
dc.date.available2023-03-21T14:42:55Z
dc.date.issued2015-08
dc.descriptionIndexación: Scopuses
dc.description.abstractBacterial regulatory networks of gene expression include the interaction of diverse types of molecules such as the small non-coding RNAs (sRNAs) and their cognate messenger RNAs (mRNAs). In this study, we demonstrated that the Salmonella Typhimurium sRNA SroC is significantly expressed between the late-exponential and stationary phase of growth in an rpoS-dependent manner. The expression of flagellar genes predicted as targets of this sRNA was quantitatively analyzed in both a ΔsroC mutant and a SroC-overexpressing (pSroC) strain. Deletion of sroC increased flagellar gene expression (i.e. flhBAE and fliE). Conversely, overexpression of SroC reduced flhBAE and fliE expression. These observations correlated with phenotypic evaluation of motility, where sroC deletion slightly increased motility, which in turn, was drastically reduced upon overexpression of SroC. The effects of deletion and overexpression of sroC in biofilm formation were also examined, where the ΔsroC and pSroC strains exhibited a reduced and increased ability to form biofilm, respectively. Furthermore, electron microscopy revealed that the wild-type strain overexpressing SroC had a non-flagellated phenotype. Taken together, our results showed that S. Typhimurium sRNA SroC modulates the flagellar synthesis by down-regulating the expression of flhBAE and fliE genes. © FEMS 2015. All rights reserved.es
dc.description.urihttps://academic-oup-com.recursosbiblioteca.unab.cl/femsle/article/362/17/fnv135/481782
dc.identifier.doi10.1093/femsle/fnv135
dc.identifier.issn0378-1097
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/47716
dc.language.isoenes
dc.publisherOxford University Presses
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectBacterial Gene Expression Regulationes
dc.subjectRiboswitches
dc.subject6S RNAes
dc.titleMotility modulation by the small non-coding RNA SroC in Salmonella Typhimuriumes
dc.typeArtículoes
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
fnv135.pdf
Tamaño:
1.29 MB
Formato:
Adobe Portable Document Format
Descripción:
FEMS Microbiology Letters Volume 362, Issue 1724 August 2015 Article number fnv135
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista