Valp1, a Newly Identified Temperate Phage Facilitating Coexistence of Lysogenic and Non-Lysogenic Populations of Vibrio anguillarum

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dc.contributor.authorArce, Manuel
dc.contributor.authorVenegas, Guillermo
dc.contributor.authorPaez, Karla
dc.contributor.authorLatz, Simone
dc.contributor.authorNavarrete, Paola
dc.contributor.authorCaruffo, Mario
dc.contributor.authorFeijoo, Carmen
dc.contributor.authorGarcía, Katherine
dc.contributor.authorBastías, Roberto
dc.date.accessioned2024-06-16T04:12:28Z
dc.date.available2024-06-16T04:12:28Z
dc.date.issued2024-04
dc.descriptionINDEXACIÓN: SCOPUS.
dc.description.abstractVibrio anguillarum is a pathogen for several fish and shellfish species. Its ecology is influenced by diverse factors, including bacteriophages. Here, we identify and characterize a new temperate bacteriophage (Valp1) of V. anguillarum. Valp1 is a myovirus with a 60 nm head and a 90 nm contractile tail. Its double-stranded DNA genome of 42,988 bp contains 68 genes, including a protelomerase gene, typical of telomeric phages. Valp1 inhibits the growth of the virulent strain of V. anguillarum PF4, while the derived lysogenic strain P1.1 presents a slight reduction in its growth but is not affected by the presence of Valp1. Both strains present similar virulence in a larval zebrafish (Danio rerio) model, and only slight differences have been observed in their biochemical profile. Co-culture assays reveal that PF4 and P1.1 can coexist for 10 h in the presence of naturally induced Valp1, with the proportion of PF4 ranging between 28% and 1.6%. By the end of the assay, the phage reached a concentration of ~108 PFU/mL, and all the non-lysogenic PF4 strains were resistant to Valp1. This equilibrium was maintained even after five successive subcultures, suggesting the existence of a coexistence mechanism between the lysogenic and non-lysogenic populations of V. anguillarum in conjunction with the phage Valp1. © 2024 by the authors.
dc.identifier.citationPathogens, Volume 13, Issue 4, April 2024, Article number 285
dc.identifier.doi10.3390/pathogens13040285
dc.identifier.issn2076-0817
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/57619
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.rights.licenseATTRIBUTION 4.0 INTERNATIONAL CC BY 4.0 Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectbacteriophage
dc.subjectphage
dc.subjecttelomeric phage
dc.subjectVibrio anguillarum
dc.subjectvibriophage
dc.subjectVibro
dc.titleValp1, a Newly Identified Temperate Phage Facilitating Coexistence of Lysogenic and Non-Lysogenic Populations of Vibrio anguillarum
dc.typeArtículo
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