Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers

Vista sencilla de ítem
dc.contributor.authorPacheco, Nicolas
dc.contributor.authorOrellana-Saez, Matias
dc.contributor.authorPepczynska, Marzena
dc.contributor.authorEnrione, Javier
dc.contributor.authorBassas-Galia, Monica
dc.contributor.authorBorrero-de Acuña, Jose M.
dc.contributor.authorZacconi, Flavia C.
dc.contributor.authorMarcoleta, Andrés E.
dc.contributor.authorPoblete-Castro, Ignacio
dc.date.accessioned2024-07-29T18:42:17Z
dc.date.available2024-07-29T18:42:17Z
dc.date.issued2019-08-01
dc.descriptionIndexación: Scopus.
dc.description.abstractExtreme environments are a unique source of microorganisms encoding metabolic capacities that remain largely unexplored. In this work, we isolated two Antarctic bacterial strains able to produce poly(3-hydroxyalkanoates) (PHAs), which were classified after 16S rRNA analysis as Pseudomonas sp. MPC5 and MPC6. The MPC6 strain presented nearly the same specific growth rate whether subjected to a temperature of 4 °C 0.18 (1/h) or 30 °C 0.2 (1/h) on glycerol. Both Pseudomonas strains produced high levels of PHAs and exopolysaccharides from glycerol at 4 °C and 30 °C in batch cultures, an attribute that has not been previously described for bacteria of this genus. The MPC5 strain produced the distinctive medium-chain-length-PHA whereas Pseudomonas sp. MPC6 synthesized a novel polyoxoester composed of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate). Batch bioreactor production of PHAs in MPC6 resulted in a titer of 2.6 (g/L) and 1.3 (g/L), accumulating 47.3% and 34.5% of the cell dry mass as PHA, at 30 and 4 °C, respectively. This study paves the way for using Antarctic Pseudomonas strains for biosynthesizing novel PHAs from low-cost substrates such as glycerol and the possibility to carry out the bioconversion process for biopolymer synthesis without the need for temperature control.
dc.description.urihttps://watermark.silverchair.com/jimb1139.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAA1QwggNQBgkqhkiG9w0BBwagggNBMIIDPQIBADCCAzYGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMpeXvPXaXI_zhiSzYAgEQgIIDB1HsE6s_pWFbnnQDa14HMWmHB_vT6UJVUS6R3adLKu2jl5ASsK7dxrKV821r8vRi9BCtSf3dN6r_WLDP9JaIHbb5MJGJVrt00lAeB3wYkyrnAXYl7gAWJe68-ksiZ9yvZSv7ltZvmu4ZZ6NoNuZzUhoB-ezwYD7qxTXxwGjU-TH4yLkrbrhUQHOABQdI8v9YsuaciR7T5xNSyKtUJJPUksKYAXRtjvWS1-h4tyyFUmvgR1LUSFNPhlfnvrrx9ruaVm1DJLhuJ6ZQsm1CLiQ9o4sJFjz8niR-zoKn27KN3KVW4vQwqQdj4UJa167XqzHWXZ2-Banc6rs--mUqNcm3x5nU_0Wq-rpmWKzfsukIuk8Iv8RnzzxQ7vFp3IfNFzaTW_HtERR2lQ5vOh07j7xK-P5M9TR2yuENTWHg6KXJFloShhD5BB4y_0Qu_iDygHWddCv651YVWzuKt_EYgpD-xdjqs9jcFrwFFGEPBKgDOOZb3tnI5sEQ-Bgw5BTOEsCMPbho-wVGUKnRr7uihTlzkgQX7vwj1sL6m4U65ZbsztB3q1eiAjr-DQcckas3i1i-v4itHMPAYWfEMMhkpoHa7N8mwWJp4HZ9Aa3uz8JirjUOR3AL2RVYMzCikOzjqFakZkEPBRk9xhknLIB-nhtcogFwZ1e_hMgo0nJ03z47mAq1a3fY0TuJpFr7ADyqaeyiY0yRuzBs5vCaXUAnmbY0132WqOassFXSzxBhGf_9pa9dtHFJ_bB9qZVisV7auftnR1aJ4CSVxB5TM6IUnqzsg78LELyDLweOe5An8sRpdmmfC-fbnX4_xzRm3Kk4kzcnPVIa7tGoNElcHoGd-2BPawH6tZy2qOvu-pYcJVZ7X4xqSQV3rf-QkG3Q_8PsWkyJ8Ut6ofZdfLQX655ubLWkumjIVJ6KteN1scd5QV4UyT7L0wm99BrYDveBcs5LpFbTnP3bEQ4jHPuDsT7WFrB6nIoXbcbemBvSv_DsqDAJPqYRUb_NEuUOnN8Qo72A4fxvyvQYSsxqcmY
dc.identifier.citationJournal of Industrial Microbiology and Biotechnology Volume 46, Issue 8, Pages 1139 - 1153 1 August 2019
dc.identifier.doi10.1007/s10295-019-02186-2
dc.identifier.issn1367-5435
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/58808
dc.language.isoen_US
dc.publisherSpringer Verlag
dc.subjectAntarctic Pseudomonas
dc.subjectExopolysaccharide
dc.subjectGlycerol
dc.subjectLow temperature
dc.subjectPoly(3-hydroxyalkanoates)
dc.subjectPsychrophiles
dc.titleExploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers
dc.typeArtículo
Archivos
Bloque original
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
jimb1139.pdf
Tamaño:
2.23 MB
Formato:
Adobe Portable Document Format
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista